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IF (Immunofluorescence)
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Goat mScarlet Polyclonal Antibody

anti-mScarlet

Reactivity
Reacts with Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Purity
This antibody is epitope-affinity purified from goat antiserum.
Synonyms
mScarlet, Antibody; anti-mScarlet; red fluorescent protein antibody; anti-mScarlet antibody
Ordering
 
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For Research Use Only!
Host
Goat
Reactivity
Reacts with Transfected cells proteins
Clonality
Polyclonal
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 26.5kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).
Purity/Purification
This antibody is epitope-affinity purified from goat antiserum.
Form/Format
Supplied as a 100 uL (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Applicable Applications for anti-mScarlet antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunoelectron Microscopy (IEM)
Application Notes
WB: 1:500-1:5,000
IF: 1:50-1:500
IHC-P: 1:50-1:500
IHC-F: 1:50-1:500
IEM: 1:50-1:500
Immunogen
Purified recombinant fluorescent protein produced in E Coli.
Note
The antibody solution should be gently mixed before use.
Preparation and Storage
For continuous use, store at 2-8 degree C for one to two days. For extended storage, store in -20 degree C freezer. Working dilution samples should be discarded if not used within 12 hours.

IF (Immunofluorescence)

(Immunofluorescence - anti-mScarlet Ab using hCEC cells transduced with mScarlet-Rab5a; cells were fixed with methanol and anti-mScarlet at 1/250;)

Application Data

(Anti-mScarlet Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)

IF (Immunofluorescence)

(Immunofluorescence in Drosophila larvae NMJ muscle 6/7 expressing mScarlet in neurons (DyGlutmScarlet) using 1st Ab anti-mScarlet at 1/1,000 and 2nd Ab anti-goat IgY conjugated to DyLight488 at 1/500;)

IF (Immunofluorescence)

(Immunofluorescence -Anti-mScarlet Ab using hCEC cells transduced with mScarlet-Rab5a; cells were fixed with methanol and Anti-mScarlet at 1/250;)

Reactivity Chart

(+++ excellent, ++ good, + poor, ND not determined)

Related Product Information for anti-mScarlet antibody
Goat polyclonal antibody to mScarlet (Scarlet fluorescent protein). mScarlet is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mScarlet is a ~26.5kDa protein that is optimally excited at a 569 nm and has a maximum of emission at 594 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
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Product Notes

The mScarlet (Catalog #AAA13885) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The anti-mScarlet reacts with Reacts with Transfected cells proteins and may cross-react with other species as described in the data sheet. AAA Biotech's mScarlet can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunoelectron Microscopy (IEM). WB: 1:500-1:5,000 IF: 1:50-1:500 IHC-P: 1:50-1:500 IHC-F: 1:50-1:500 IEM: 1:50-1:500. Researchers should empirically determine the suitability of the mScarlet for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mScarlet, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.