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ICC (Immunocytochemistry)
(Anti- MSH2 Picoband antibody, AAA46503,ICCICC: SMMC Cell)
MSH2 Polyclonal Antibody | anti-MSH2 antibody
Anti-MSH2 Antibody
Gene Names
MSH2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
MSH2, Antibody; Anti-MSH2 Antibody; DNA mismatch repair protein Msh2; BAT26; COCA 1; COCA1; FCC 1; FCC1; hMSH2; HNPCC 1; HNPCC; HNPCC1; LCFS2; MSH 2; Msh2; MSH2_HUMAN; MutS homolog 2; MutS homolog 2 colon cancer nonpolyposis type 1; MutS protein homolog 2; mutS homolog 2; anti-MSH2 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
934
Applicable Applications for anti-MSH2 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human MSH2 recombinant protein (Position: Q337-N583). Human MSH2 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat MSH2, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ICC (Immunocytochemistry)
(Anti- MSH2 Picoband antibody, AAA46503,ICCICC: SMMC Cell)
ICC (Immunocytochemistry)
(Anti- MSH2 Picoband antibody, AAA46503,ICCICC: SMMC Cell)
IHC (Immunohistochemistry)
(Anti- MSH2 Picoband antibody, AAA46503, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemistry)
(Anti- MSH2 Picoband antibody, AAA46503, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- MSH2 Picoband antibody, AAA46503, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohistochemisry)
(Anti- MSH2 Picoband antibody, AAA46503, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti- MSH2 Picoband antibody, AAA46503, IHC(P)IHC(P): Mouse Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti- MSH2 Picoband antibody, AAA46503, IHC(P)IHC(P): Mouse Intestine Tissue)
WB (Western Blot)
(Anti- MSH2 Picoband antibody, AAA46503, Western blottingAll lanes: Anti MSH2 (AAA46503) at 0.5ug/mlLane 1: Mouse Testis Tissue Lysate at 50ugLane 2: Mouse Skeletal Muscle Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugPredicted bind size: 105KDObserved bind size: 105KD)
WB (Western Blot)
(Anti- MSH2 Picoband antibody, AAA46503, Western blottingAll lanes: Anti MSH2 (AAA46503) at 0.5ug/mlLane 1: Mouse Testis Tissue Lysate at 50ugLane 2: Mouse Skeletal Muscle Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugPredicted bind size: 105KDObserved bind size: 105KD)
Related Product Information for anti-MSH2 antibody
Description: Rabbit IgG polyclonal antibody for DNA mismatch repair protein Msh2(MSH2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Background: DNA mismatch repair protein Msh2, also known as MutS protein homolog 2 or MSH2, is a protein that in humans is encoded by the MSH2 gene, which is located on chromosome 2. MSH2 is a tumor suppressor gene and more specifically a caretaker gene that codes for a DNA mismatch repair (MMR) protein, MSH2 which forms aheterodimer with MSH6 to make the human MutSalpha mismatch repair complex. It also dimerizes with MSH3 to form the MutSbeta DNA repair complex. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. It has been found that MSH2 may also be a coactivator of ESR1-dependent gene expression.
Background: DNA mismatch repair protein Msh2, also known as MutS protein homolog 2 or MSH2, is a protein that in humans is encoded by the MSH2 gene, which is located on chromosome 2. MSH2 is a tumor suppressor gene and more specifically a caretaker gene that codes for a DNA mismatch repair (MMR) protein, MSH2 which forms aheterodimer with MSH6 to make the human MutSalpha mismatch repair complex. It also dimerizes with MSH3 to form the MutSbeta DNA repair complex. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. It has been found that MSH2 may also be a coactivator of ESR1-dependent gene expression.
References
1. de Wind N, Dekker M, Berns A, Radman M, te Riele H (July 1995). "Inactivation of the mouse Msh2 gene results in mismatch repair deficiency, methylation tolerance, hyperrecombination, and predisposition to cancer". Cell 82 (2): 321-30. 2. Mellon I, Rajpal DK, Koi M, Boland CR, Champe GN (April 1996). "Transcription-coupled repair deficiency and mutations in human mismatch repair genes". Science 272 (5261): 557-60. 3. Wada-Hiraike, O., Yano, T., Nei, T., Matsumoto, Y., Nagasaka, K., Takizawa, S., Oishi, H., Arimoto, T., Nakagawa, S., Yasugi, T., Kato, S., Taketani, Y. The DNA mismatch repair gene hMSH2 is a potent coactivator of oestrogen receptor-alpha. Brit. J. Cancer 92: 2286-2291, 2005.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,323 Da
NCBI Official Full Name
DNA mismatch repair protein Msh2 isoform 1
NCBI Official Synonym Full Names
mutS homolog 2
NCBI Official Symbol
MSH2
NCBI Official Synonym Symbols
FCC1; COCA1; HNPCC; LCFS2; HNPCC1
NCBI Protein Information
DNA mismatch repair protein Msh2
UniProt Protein Name
DNA mismatch repair protein Msh2
UniProt Gene Name
MSH2
UniProt Synonym Gene Names
hMSH2
UniProt Entry Name
MSH2_HUMAN
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Product Notes
The MSH2 msh2 (Catalog #AAA46503) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-MSH2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MSH2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MSH2 msh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MSH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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