FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using MTBP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit MTBP Polyclonal Antibody | anti-MTBP antibody
MTBP Antibody (C-term)
Gene Names
MTBP; MDM2BP
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MTBP, Antibody; MTBP Antibody (C-term); Mdm2-binding protein; hMTBP; MTBP; anti-MTBP antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MTBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 853-881 amino acids from the C-terminal region of human MTBP.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
853-881
Sequence Length
329
Applicable Applications for anti-MTBP antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using MTBP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells using MTBP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with MTBP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with MTBP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of MTBP antibody (C-term) in K562 cell line lysates (35ug/lane). MTBP (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of MTBP antibody (C-term) in K562 cell line lysates (35ug/lane). MTBP (arrow) was detected using the purified Pab.)
Related Product Information for anti-MTBP antibody
MTBP inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells. The protein may play a role in MDM2-dependent TP53/p53 homeostasis in unstressed cells. The protein inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of TP53/p53 and its subsequent degradation.
Product Categories/Family for anti-MTBP antibody
References
Brady,M., Mol. Cell. Biol. 25 (2), 545-553 (2005)
Boyd,M.T., J. Biol. Chem. 275 (41), 31883-31890 (2000)
Boyd,M.T., J. Biol. Chem. 275 (41), 31883-31890 (2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
102193
NCBI Official Full Name
mdm2-binding protein
NCBI Official Synonym Full Names
MDM2 binding protein
NCBI Official Symbol
MTBP
NCBI Official Synonym Symbols
MDM2BP
NCBI Protein Information
mdm2-binding protein
UniProt Protein Name
Mdm2-binding protein
UniProt Gene Name
MTBP
UniProt Synonym Gene Names
hMTBP
UniProt Entry Name
MTBP_HUMAN
Similar Products
Product Notes
The MTBP mtbp (Catalog #AAA288883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 853-881. The MTBP Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's MTBP can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the MTBP mtbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.