Goat NALP3 / NLRP3 Polyclonal Antibody | anti-NALP3 antibody

Goat Polyclonal to Human NALP3 / NLRP3

Cat. Num. AAA50832

• Gene Names: NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
• Reactivity: Human, Monkey
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
• Synonyms: NALP3 / NLRP3, Antibody; Goat Polyclonal to Human NALP3 / NLRP3; Anti-NALP3 / NLRP3 Antibody (Internal) IHC-plus; NLRP3; AGTAVPRL; AII; C1orf7; Caterpiller protein 1.1; Cryopyrin; FCAS; FCU; PYPAF1; NALP3; CIAS1; CLR1.1; MWS; Human NALP3; anti-NALP3 antibody

• Host: Goat
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Specificity: Human NLRP3. This antibody is expected to recognize all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1036

• Applicable Applications for anti-NALP3 antibody: ELISA, IHC (Immunohistochemistry)
• Immunogen Species: Human
• Epitope: aa82-93
• Immunogen: NALP3 / NLRP3 antibody was raised against peptide with sequence C-DLYEKAKRDEPK from the internal region of the protein sequence according to NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Usage: Immunohistochemistry: MBS241660 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat enduced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathlogist to confirm staining specificity. The optimal working concentration for MBS241660 was determined to be 2.5 ug/ml
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

IHC (Immunohistochemistry)

(Anti-NLRP3 antibody IHC of human peripheral blood leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 34878693
• NCBI GeneID: 114548
• NCBI Accession #: NP_004886.3
• NCBI GenBank Nucleotide #: NM_004895.4
• UniProt Accession #: Q96P20; O75434; Q17RS2; Q59H68; Q5JQS8; Q5JQS9; Q6TG35; Q8TCW0; B2RC97; B7ZKS9; B7ZKT2; B7ZKT3
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 3 isoform a
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 3
• NCBI Official Symbol: NLRP3
• NCBI Official Synonym Symbols: AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 3; cryopyrin; caterpiller protein 1.1; PYRIN-containing APAF1-like protein 1; NACHT, LRR and PYD containing protein 3; cold autoinflammatory syndrome 1 protein; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 3
• UniProt Gene Name: NLRP3
• UniProt Synonym Gene Names: C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1
• UniProt Entry Name: NALP3_HUMAN