WB (Western Blot)
(Western blot analysis of brain using NCSTN antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit NCSTN Polyclonal Antibody | anti-NCSTN antibody
NCSTN Antibody
Gene Names
NCSTN; ATAG1874
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NCSTN, Antibody; NCSTN Antibody; Anterior pharynx defective 2; APH 2; APH2; ATAG1874; KIAA0253; Ncstn; NCT; NICA_HUMAN; Nicastrin; RP11 517F10.1; RP11517F101; anti-NCSTN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NCSTN antibody detects endogenous levels of total NCSTN
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
689
Applicable Applications for anti-NCSTN antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human NCSTN
Subcellular Location
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity
Detected in brain (at protein level) (PubMed:10993067). Widely expressed (PubMed:11396676).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (83%), Bovine (83%), Horse (83%), Sheep (83%), Rabbit (100%), Dog (83%), Xenopus (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of brain using NCSTN antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of brain using NCSTN antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry)
(AAA326023 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA326023 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NCSTN antibody
Description: Nicastrin is a transmembrane glycoprotein serving as an essential component of the gamma-secretase complex (1, 2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound gamma-secretase complex (3). Nicastrin also serves as a docking site for gamma-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to gamma-secretase to ensure the correct cleavage process (2, 4).
Function: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:10993067, PubMed:12679784, PubMed:25043039, PubMed:26280335). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.
Subunit Structure: Component of the gamma-secretase complex (PubMed:10993067). The functional gamma-secretase complex is composed of at least four polypeptides: a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2 (PubMed:12740439, PubMed:25043039, PubMed:26623517, PubMed:26280335, PubMed:25918421). Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP) (PubMed:10993067). Interacts with PSEN1 and PSEN2 (PubMed:10993067).
Post-translational Modifications: N-glycosylated.
Similarity: Belongs to the nicastrin family.
Function: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:10993067, PubMed:12679784, PubMed:25043039, PubMed:26280335). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.
Subunit Structure: Component of the gamma-secretase complex (PubMed:10993067). The functional gamma-secretase complex is composed of at least four polypeptides: a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2 (PubMed:12740439, PubMed:25043039, PubMed:26623517, PubMed:26280335, PubMed:25918421). Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP) (PubMed:10993067). Interacts with PSEN1 and PSEN2 (PubMed:10993067).
Post-translational Modifications: N-glycosylated.
Similarity: Belongs to the nicastrin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 77 kDa
Predicted: 79 kDa
Predicted: 79 kDa
NCBI Official Full Name
nicastrin isoform 2
NCBI Official Synonym Full Names
nicastrin
NCBI Official Symbol
NCSTN
NCBI Official Synonym Symbols
ATAG1874
NCBI Protein Information
nicastrin
UniProt Protein Name
Nicastrin
UniProt Gene Name
NCSTN
UniProt Synonym Gene Names
KIAA0253
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Product Notes
The NCSTN ncstn (Catalog #AAA326023) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCSTN Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NCSTN can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NCSTN ncstn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCSTN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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