Rabbit Nicastrin Polyclonal Antibody | anti-NCSTN antibody

Nicastrin Antibody

Cat. Num. AAA40197

• Gene Names: NCSTN; ATAG1874
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: Nicastrin Antibody is affinity chromatography purified via peptide column.
• Synonyms: Nicastrin, Antibody; Nicastrin Antibody; Nicastrin; ATAG1874; KIAA0253; UNQ1874/PRO4317; nicastrin; anti-NCSTN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Nicastrin Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 709

• Applicable Applications for anti-NCSTN antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: Nicastrin antibody was raised against a 18 amino acid synthetic peptide from near the center of human Nicastrin.
• Buffer: Nicastrin Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Nicastrin antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of Nicastrin in mouse brain tissue with Nicastrin antibody at 20 μg/ml.Green: Nicastrin Antibody (3985)Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of Nicastrin in human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1μg/mL.)

Related Product Information for anti-NCSTN antibody

Nicastrin Antibody: Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality.

NCBI and Uniprot Product Information

• NCBI GI #: 24638433
• NCBI GeneID: 23385
• NCBI Accession #: NP_056146
• NCBI GenBank Nucleotide #: NM_015331.2
• UniProt Accession #: Q92542; Q5T207; Q5T208; Q86VV5
• Molecular Weight: 76,744 Da
• NCBI Official Full Name: nicastrin isoform 1
• NCBI Official Synonym Full Names: nicastrin
• NCBI Official Symbol: NCSTN
• NCBI Official Synonym Symbols: ATAG1874
• NCBI Protein Information: nicastrin; anterior pharynx-defective 2
• UniProt Protein Name: Nicastrin
• UniProt Gene Name: NCSTN
• UniProt Synonym Gene Names: KIAA0253
• UniProt Entry Name: NICA_HUMAN