WB (Western Blot)
(Detection of human Nicastrin by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody AAA212710 (lot AAA212710-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human Nicastrin Polyclonal Antibody | anti-NCSTN antibody
Rabbit anti-Nicastrin Antibody, Affinity Purified
Gene Names
NCSTN; ATAG1874
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Nicastrin, Antibody; Rabbit anti-Nicastrin Antibody, Affinity Purified; anterior pharynx-defective 2; ATAG1874; nicastrin; Nicastrin; anti-NCSTN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NCSTN antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 659 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Nicastrin by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody AAA212710 (lot AAA212710-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human Nicastrin by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody AAA212710 (lot AAA212710-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Nicastrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody AAA212710 (lot AAA212710-1) used for IP at 6 ug per reaction. For blotting immunoprecipitated Nicastrin, AAA212710 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Nicastrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody AAA212710 (lot AAA212710-1) used for IP at 6 ug per reaction. For blotting immunoprecipitated Nicastrin, AAA212710 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-NCSTN antibody
Nicastrin is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92542].
Product Categories/Family for anti-NCSTN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76,744 Da
NCBI Official Full Name
nicastrin isoform 1
NCBI Official Synonym Full Names
nicastrin
NCBI Official Symbol
NCSTN
NCBI Official Synonym Symbols
ATAG1874
NCBI Protein Information
nicastrin; anterior pharynx-defective 2
UniProt Protein Name
Nicastrin
UniProt Gene Name
NCSTN
UniProt Synonym Gene Names
KIAA0253
UniProt Entry Name
NICA_HUMAN
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Product Notes
The NCSTN ncstn (Catalog #AAA212710) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Nicastrin Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nicastrin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NCSTN ncstn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nicastrin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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