Rabbit NDUFB7 Polyclonal Antibody | anti-NDUFB7 antibody

Anti-NDUFB7 Antibody, Rabbit Polyclonal

Cat. Num. AAA256575

• Reactivity: Human, Cynomolgus
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NDUFB7, Antibody; Anti-NDUFB7 Antibody, Rabbit Polyclonal; NDUFB7 Antibody, Rabbit PAb, Antigen Affinity Purified; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa; Anti-B18 Antibody; Anti-CI-B18 Antibody; anti-NDUFB7 antibody

• Host: Rabbit
• Reactivity: Human, Cynomolgus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NDUFB7
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NDUFB7 antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human NDUFB7 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human NDUFB7 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(NDUFB7 was immunoprecipitated using:Lane A:0.5 mg HL60 Whole Cell Lysate4 uL anti-NDUFB7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NDUFB7 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 16 kDaObserved band size :20 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of NDUFB7 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NDUFB7 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

IHC (Immunohistochemisry)

(Immunochemical staining of human NDUFB7 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human NDUFB7 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-NDUFB7 rabbit polyclonal antibody at 1:500 dilutionLane A: PC3 Whole Cell LysateLane B: HL60 Whole Cell LysateLane C: A431 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:16 kDaObserved band size:19 kDa)

Related Product Information for anti-NDUFB7 antibody

NDUFB7 (NADH: Ubiquinone Oxidoreductase Subunit B7) is a Protein Coding gene. The NDUFB7 gene, located on 19p13.12, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. NDUFB7 belongs to the complex I NDUFB7 subunit family. The protein encoded by this gene is a subunit of the multisubunit NADH: ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Diseases associated with NDUFB7 include Codas Syndrome and Mitochondrial Dna Depletion Syndrome 9. Among its related pathways are GABAergic synapse and Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.

References

Wong YC, et al. (1990) Cdna cloning of a novel cell adhesion protein expressed in human squamous carcinoma cells. Biochem Biophys Res Commun 166 (2): 984-992.Triepels R, et al. (2000) Characterization of the human complex i ndufb7 and 17.2-kda cdnas and mutational analysis of 19 genes of the hp fraction in complex i-deficient-patients. Hum Genet 106 (4): 385-391.Smeitink J, et al. (1999) Human mitochondrial complex i in health and disease. Am J Hum Genet 64 (6): 1505-1510.