Rabbit NF45/ILF2 Polyclonal Antibody | anti-NF45/ILF2 antibody

Anti-NF45/ILF2 Antibody Picoband

Cat. Num. AAA19816

• Gene Names: ILF2; NF45; PRO3063
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Immunogen affinity purified.
• Synonyms: NF45/ILF2, Antibody; Anti-NF45/ILF2 Antibody Picoband; Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody; anti-NF45/ILF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Expressed in all tissues.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Applicable Applications for anti-NF45/ILF2 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 0.1-0.25ug/ml, Human, Monkey, Mouse, Rat; IHC: 1-2ug/ml, Human, Mouse, Rat; ICC/IF: 5ug/ml, Human; FC/FACS (Fixed): 1-3ug /1x10⁶ cells, Human; ELISA: 0.1-0.5ug/ml
• Immunogen: E coli-derived human Pyruvate Carboxylase/PC recombinant protein (Position: R88-D1165). Human PC shares 97% and 96.6% amino acid (aa) sequence identity with mouse and rat PC, respectively.
• Subcellular Localization: Cytoplasm, Nucleus
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
• Cross Reactivity: No cross reactivity with other proteins.
• Protein Function: Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.
• Preparation and Storage: At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 10. Flow Cytometry analysis of JK cells using anti-NF45/ILF2 antibody (AAA19816).Overlay histogram showing JK cells stained with AAA19816 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF45/ILF2 Antibody (AAA19816, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence)

(Figure 9. IF analysis of NF45/ILF2 using anti-NF45/ILF2 antibody (AAA19816) and anti-Beta Tubulin antibody (M01857-3).NF45/ILF2 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: monkey COS-7 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat C6 whole cell lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF45/ILF2 antigen affinity purified polyclonal antibody (#AAA19816) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NF45/ILF2 at approximately 43 kDa. The expected band size for NF45/ILF2 is at 43 kDa.)

Related Product Information for anti-NF45/ILF2 antibody

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Categories/Family for anti-NF45/ILF2 antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins

References

1. Kao, P. N., Chen, L., Brock, G., Ng, J., Kenny, J., Smith, A. J., Corthesy, B. Cloning and expression of cyclosporin A- and FK506-sensitive nuclear factor of activated T-cells: NF45 and NF90. J. Biol. Chem. 269: 20691-20699, 1994.

NCBI and Uniprot Product Information

• NCBI GI #: 24234747
• NCBI GeneID: 3608
• NCBI Accession #: NP_004506.2
• NCBI GenBank Nucleotide #: NM_004515.3
• UniProt Accession #: Q12905; Q5SR10; Q5SR11; Q7L7R3; Q9BWD4; Q9P1N0; A6NDB0; B2R8G7
• Molecular Weight: 43,062 Da
• NCBI Official Full Name: interleukin enhancer-binding factor 2 isoform 1
• NCBI Official Synonym Full Names: interleukin enhancer binding factor 2
• NCBI Official Symbol: ILF2
• NCBI Official Synonym Symbols: NF45; PRO3063
• NCBI Protein Information: interleukin enhancer-binding factor 2; interleukin enhancer binding factor 2, 45kD; nuclear factor of activated T-cells, 45-kDa; interleukin enhancer binding factor 2, 45kDa
• UniProt Protein Name: Interleukin enhancer-binding factor 2
• UniProt Gene Name: ILF2
• UniProt Synonym Gene Names: NF45
• UniProt Entry Name: ILF2_HUMAN