WB (Western Blot)
(Anti-NFkB p100/p52 antibody, AAA45403, Western blottingLane 1: Mouse Liver Tissue LysateLane 2: HEPA Cell Lysate)
Rabbit anti-Mouse, Rat NFkB p100/p52 Polyclonal Antibody | anti-NFKB2 antibody
Anti-NFkB p100/p52 antibody
Gene Names
Nfkb2; lyt; p49; p52; p50B; p49/p100; NF-kappaB2
Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
NFkB p100/p52, Antibody; Anti-NFkB p100/p52 antibody; Nuclear factor NF-kappa-B p100 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2(p49/p100); DNA binding factor KBF2 antibody; DNA-binding factor KBF2 antibody; H2TF1 antibody; Lymphocyte translocation chromosome 10 antibody; Lymphocyte translocation chromosome 10 protein antibody; Lyt 10 antibody; Lyt10 antibody; NF kB2 antibody; NFKB2 antibody; NFKB2_HUMAN antibody; Nuclear factor NF kappa B p100 subunit antibody; Nuclear factor NF kappa B p52 subunit antibody; Nuclear factor NF-kappa-B p52 subunit antibody; Nuclear factor of kappa light chain gene enhancer in B cells 2 antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 antibody; Oncogene Lyt 10 antibody; Oncogene Lyt-10 antibody; p105 antibody; p49/p100 antibody; anti-NFKB2 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
899
Applicable Applications for anti-NFKB2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse NFkB p100 (5-21aa YDPGLDGIPEYDDFEFS), identical to the related rat sequence.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-NFkB p100/p52 antibody, AAA45403, Western blottingLane 1: Mouse Liver Tissue LysateLane 2: HEPA Cell Lysate)
WB (Western Blot)
(Anti-NFkB p100/p52 antibody, AAA45403, Western blottingLane 1: Mouse Liver Tissue LysateLane 2: HEPA Cell Lysate)
IHC (Immunohistochemistry)
(Anti-NFkB p100/p52 antibody, AAA45403, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohistochemistry)
(Anti-NFkB p100/p52 antibody, AAA45403, IHC(P)IHC(P): Rat Intestine Tissue)
Related Product Information for anti-NFKB2 antibody
Description: Rabbit IgG polyclonal antibody for Nuclear factor NF-kappa-B p100 subunit(NFKB2) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: NFKB2(Nuclear Factor Kappa-B, Subunit 2), also known as NFKB or p52/p100 SUBUNIT, is a protein that in humans is encoded by the NFKB2 gene. Liptay et al.(1992) mapped the gene for what they called the p49/p100 subunit of NFKB(NFKB2) to chromosome 10 by Southern blot analysis of panels of human/Chinese hamster cell hybrids. By fluorescence in situ hybridization(FISH), they confirmed the localization and mapped the gene with greater resolution to 10q24. NFKB2 appears to be the same as LYT10. Claudio et al.(2002) showed that bone marrow(BM) cells from Nfkb2-deficient mice, but not Nfkb1-deficient mice, failed to increase relative and total IgD-positive transitional-1(T1) stage B cells in response to Baff. In vivo, however, Nfkb2-deficient mice did generate mature B cells, but at reduced numbers. Mice of the aly/aly strain, which are naturally deficient in Nik, and mice of the A/WySNJ strain, which have a mutation in Baffr, also failed to produce T1 B cells in response to Baff. Baff stimulation enhanced expression of Bcl2 in T1 B cells, thereby promoting B-cell survival, and caused the processing of the p100 form of Nfkb2 to p52, which again required Baffr and Nik, but not Nemo(IKKG). Immunoblot analysis showed that BM cells contained primarily p100.
Background: NFKB2(Nuclear Factor Kappa-B, Subunit 2), also known as NFKB or p52/p100 SUBUNIT, is a protein that in humans is encoded by the NFKB2 gene. Liptay et al.(1992) mapped the gene for what they called the p49/p100 subunit of NFKB(NFKB2) to chromosome 10 by Southern blot analysis of panels of human/Chinese hamster cell hybrids. By fluorescence in situ hybridization(FISH), they confirmed the localization and mapped the gene with greater resolution to 10q24. NFKB2 appears to be the same as LYT10. Claudio et al.(2002) showed that bone marrow(BM) cells from Nfkb2-deficient mice, but not Nfkb1-deficient mice, failed to increase relative and total IgD-positive transitional-1(T1) stage B cells in response to Baff. In vivo, however, Nfkb2-deficient mice did generate mature B cells, but at reduced numbers. Mice of the aly/aly strain, which are naturally deficient in Nik, and mice of the A/WySNJ strain, which have a mutation in Baffr, also failed to produce T1 B cells in response to Baff. Baff stimulation enhanced expression of Bcl2 in T1 B cells, thereby promoting B-cell survival, and caused the processing of the p100 form of Nfkb2 to p52, which again required Baffr and Nik, but not Nemo(IKKG). Immunoblot analysis showed that BM cells contained primarily p100.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
96,832 Da
NCBI Official Full Name
Nuclear factor NF-kappa-B p100 subunit
NCBI Official Synonym Full Names
nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100
NCBI Official Symbol
Nfkb2
NCBI Official Synonym Symbols
lyt; p49; p52; p50B; p49/p100; NF-kappaB2
NCBI Protein Information
nuclear factor NF-kappa-B p100 subunit; NF kappaB2; DNA-binding factor KBF2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100
UniProt Protein Name
Nuclear factor NF-kappa-B p100 subunit
UniProt Gene Name
Nfkb2
UniProt Entry Name
NFKB2_MOUSE
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Product Notes
The NFKB2 nfkb2 (Catalog #AAA45403) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NFkB p100/p52 antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NFkB p100/p52 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NFKB2 nfkb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB p100/p52, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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