Rabbit Nhe-1 Polyclonal Antibody | anti-SLC9A1 antibody

Nhe-1 Antibody

Cat. Num. AAA39704

• Gene Names: SLC9A1; APNH; NHE1; NHE-1; PPP1R143
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: Nhe-1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Nhe-1, Antibody; Nhe-1 Antibody; Nhe-1; APNH; NHE1; NHE-1; APNH1; solute carrier family 9 (sodium/hydrogen exchanger), member 1; anti-SLC9A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Nhe-1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 815

• Applicable Applications for anti-SLC9A1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: Nhe-1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human Nhe-1.
• Buffer: Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Nhe-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of Nhe-1 in Human Brain cells with Nhe-1 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemical staining of human brain tissue using Nhe-1 antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of Nhe-1 in MOLT4 cell lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 μg/mL.)

Related Product Information for anti-SLC9A1 antibody

Nhe-1 Antibody: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 127809
• NCBI GeneID: 6548
• UniProt Accession #: P19634; Q96EM2; B1ALD6; D3DPL4
• Molecular Weight: 61,013 Da
• NCBI Official Full Name: Sodium/hydrogen exchanger 1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
• NCBI Official Symbol: SLC9A1
• NCBI Official Synonym Symbols: APNH; NHE1; NHE-1; PPP1R143
• NCBI Protein Information: sodium/hydrogen exchanger 1; Na(+)/H(+) exchanger 1; Na-Li countertransporter; protein phosphatase 1, regulatory subunit 143; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive)
• UniProt Protein Name: Sodium/hydrogen exchanger 1
• UniProt Gene Name: SLC9A1
• UniProt Synonym Gene Names: APNH1; NHE1; NHE-1
• UniProt Entry Name: SL9A1_HUMAN