WB (Western Blot)
(Anti-NKX6-2 Antibody (N-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
Rabbit anti-Human NKX6-2 Polyclonal Antibody | anti-NKX6-2 antibody
NKX6-2 Antibody (N-term)
Gene Names
NKX6-2; GTX; NKX6B; NKX6.2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
NKX6-2, Antibody; NKX6-2 Antibody (N-term); Homeobox protein Nkx-62; Homeobox protein NK-6 homolog B; NKX6-2; GTX; NKX6B; anti-NKX6-2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NKX6-2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-43 amino acids from the N-terminal region of human NKX6-2.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
6-2
Sequence Length
277
Applicable Applications for anti-NKX6-2 antibody
ELISA, WB (Western Blot)
Function
Transcription factor with repressor activity involved in the regulation of axon-glial interactions at myelin paranodes in oligodendrocytes. Binds to the consensus DNA sequence 5'-(A/T)TTAATGA- 3'. In oligodendrocytes, binds to MBP and PLP1 promoter regions.
Cellular Location
Nucleus {ECO:0000250|UniProtKB:D3Z4R4}.
Tissue Location
Highest expression in brain.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Anti-NKX6-2 Antibody (N-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
WB (Western Blot)
(Anti-NKX6-2 Antibody (N-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
WB (Western Blot)
(NKX6-2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NKX6-2 antibody detected the NKX6-2 protein (arrow).)
WB (Western Blot)
(NKX6-2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NKX6-2 antibody detected the NKX6-2 protein (arrow).)
Related Product Information for anti-NKX6-2 antibody
The function of this protein remains unknown.
References
Lee, S.H., et al. Mamm. Genome 12(2):157-162(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29263
NCBI Official Full Name
homeobox protein Nkx-6.2
NCBI Official Synonym Full Names
NK6 homeobox 2
NCBI Official Symbol
NKX6-2
NCBI Official Synonym Symbols
GTX; NKX6B; NKX6.2
NCBI Protein Information
homeobox protein Nkx-6.2
UniProt Protein Name
Homeobox protein Nkx-6.2
UniProt Gene Name
NKX6-2
UniProt Synonym Gene Names
GTX; NKX6B
UniProt Entry Name
NKX62_HUMAN
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Product Notes
The NKX6-2 nkx6-2 (Catalog #AAA285441) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 6-2. The NKX6-2 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NKX6-2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NKX6-2 nkx6-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKX6-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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