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product-image-AAA59999_WB13.jpg WB (Western Blot) (NonO antibody (pAb) tested by Western blot. Whole cell extract of HeLa cells (20 ug) probed with NonO antibody (1 ug/ml dilution).)

Rabbit anti-Human NonO Polyclonal Antibody | anti-NonO antibody

NonO antibody (pAb)

Gene Names
NONO; P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
NonO, Antibody; NonO antibody (pAb); Non-POU Domain Containing; Octamer-Binding; 54 KDa Nuclear RNA- And DNA-Binding Protein; P54; NMT55; NRB54; P54NRB; anti-NonO antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-NonO antibody
WB (Western Blot), IP (Immunoprecipitation)
Immunogen
This NonO antibody was raised against a peptide corresponding to the C-terminus of human NonO.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

WB (Western Blot)

(NonO antibody (pAb) tested by Western blot. Whole cell extract of HeLa cells (20 ug) probed with NonO antibody (1 ug/ml dilution).)

product-image-AAA59999_WB13.jpg WB (Western Blot) (NonO antibody (pAb) tested by Western blot. Whole cell extract of HeLa cells (20 ug) probed with NonO antibody (1 ug/ml dilution).)

WB (Western Blot)

(NonO antibody (pAb) tested by Immunoprecipitation. 5 ug of NonO antibody was used to immunoprecipitate NonO from 400 ug of HeLa whole cell extract (lane 2). 5 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the NonO antibody at a dilution of 1 ug/ml.)

product-image-AAA59999_WB15.jpg WB (Western Blot) (NonO antibody (pAb) tested by Immunoprecipitation. 5 ug of NonO antibody was used to immunoprecipitate NonO from 400 ug of HeLa whole cell extract (lane 2). 5 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the NonO antibody at a dilution of 1 ug/ml.)
Related Product Information for anti-NonO antibody
Short Description: Our NonO antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.

Background: NonO (non-POU domain containing, octamer-binding, p54(nrb), NRB54, NMT55) is a DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. This gene also encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma.
Product Categories/Family for anti-NonO antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52kDa
NCBI Official Full Name
non-POU domain-containing octamer-binding protein isoform 1
NCBI Official Synonym Full Names
non-POU domain containing octamer binding
NCBI Official Symbol
NONO
NCBI Official Synonym Symbols
P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
NCBI Protein Information
non-POU domain-containing octamer-binding protein
UniProt Protein Name
Non-POU domain-containing octamer-binding protein
UniProt Gene Name
NONO
UniProt Synonym Gene Names
NRB54; NonO protein; p54nrb
UniProt Entry Name
NONO_HUMAN

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Product Notes

The NonO nono (Catalog #AAA59999) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NonO antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NonO can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the NonO nono for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NonO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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