IF (Immunofluorescence)
(Immunofluorescence staining of NOTCH1 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NOTCH1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Rabbit NOTCH1 Polyclonal Antibody | anti-NOTCH1 antibody
Anti-NOTCH1 Antibody, Rabbit Polyclonal
Gene Names
NOTCH1; hN1; TAN1
Reactivity
HumanPredicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
NOTCH1, Antibody; Anti-NOTCH1 Antibody, Rabbit Polyclonal; NOTCH1 Antibody, Rabbit PAb, Antigen Affinity Purified; notch 1; Anti-AOS5 Antibody; Anti-AOVD1 Antibody; Anti-hN1 Antibody; Anti-TAN1 Antibody; anti-NOTCH1 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NOTCH1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NOTCH1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human NOTCH1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human NOTCH1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of NOTCH1 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NOTCH1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of NOTCH1 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NOTCH1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
IHC (Immunohistochemistry)
(Immunochemical staining of human NOTCH1 in human ovarian cancer with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human NOTCH1 in human ovarian cancer with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-NOTCH1 antibody
NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair the regeneration of skeletal muscles.NOTCH1 is a prevalent signaling pathway in T cell acute lymphoblastic leukemia (T-ALL). The NOTCH signaling pathway is a conserved signaling cascade that regulates many aspects of development and homeostasis in multiple organ systems. The proto-oncogene NOTCH1 is frequently mutated in around 10% of patients with chronic lymphocytic leukemia (CLL). NOTCH1 mutations in oral squamous cell carcinoma (OSCC) frequently occur near the ligand-binding region. These mutations change the domain structure of this protein and affect the ligand-binding activity. When NOTCH1 is activated by ligand binding, the NOTCH1 intracellular domain (NICD) is cleaved from the cell membrane.
Product Categories/Family for anti-NOTCH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
272,505 Da
NCBI Official Full Name
neurogenic locus notch homolog protein 1 preproprotein
NCBI Official Synonym Full Names
notch 1
NCBI Official Symbol
NOTCH1
NCBI Official Synonym Symbols
hN1; TAN1
NCBI Protein Information
neurogenic locus notch homolog protein 1; Notch homolog 1, translocation-associated; translocation-associated notch protein TAN-1
UniProt Protein Name
Neurogenic locus notch homolog protein 1
UniProt Gene Name
NOTCH1
UniProt Synonym Gene Names
TAN1; Notch 1; hN1; NICD
UniProt Entry Name
NOTC1_HUMAN
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Product Notes
The NOTCH1 notch1 (Catalog #AAA254175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOTCH1 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's NOTCH1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NOTCH1 notch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOTCH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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