Rabbit NRM/Nurim Polyclonal Antibody | anti-NRM antibody

Anti-NRM/Nurim Antibody, Rabbit Polyclonal

Cat. Num. AAA255102

• Gene Names: NRM; NRM29
• Reactivity: Human; Predicted to React with: Pig (Species predicted to react based on 100% sequence homology)
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NRM/Nurim, Antibody; Anti-NRM/Nurim Antibody, Rabbit Polyclonal; NRM/Nurim Antibody, Rabbit PAb, Antigen Affinity Purified; nurim (nuclear envelope membrane protein); Anti-NRM29 Antibody; anti-NRM antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Pig (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NRM/Nurim
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NRM antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the center region of the Human NRM/Nurim
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human NRM/Nurim, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(NRM was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-NRM rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NRM rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :34 kDa)

IHC (Immunohiostchemistry)

(Immunochemical staining of human NRM in human stomach with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-NRM rabbit polyclonal antibody at 1:2000 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:32 kDa)

Related Product Information for anti-NRM antibody

NRM (Nurim) is a Protein Coding gene. The protein encoded by this gene contains transmembrane domains and resides within the inner nuclear membrane (INM), where it is tightly associated with the nucleus. Nurim is a multispanning membrane protein without large hydrophilic domains that is very tightly associated with the nucleus. Unlike the known nuclear envelope (NE) membrane proteins, it is neither associated with nuclear pores nor targeted like lamin-associated membrane proteins. This protein shares homology with isoprenylcysteine carboxy methyltransferase enzymes. Alternative splicing results in multiple transcript variants that encode different protein isoforms. Nurim lacks an N-terminal domain characteristic of other INM proteins examined to date and may represent a class of proteins that localize to the INM by a distinct mechanism.

References

Rolls MM, et al. (1999) A visual screen of a gfp-fusion library identifies a new type of nuclear envelope membrane protein. J Cell Biol 146 (1): 29-44.Hofemeister H, et al. (2005) Analysis of the localization and topology of nurim, a polytopic protein tightly associated with the inner nuclear membrane. J Biol Chem 280 (4): 2512-2521.Chen H, et al. (2012) The integral nuclear membrane protein nurim plays a role in the suppression of apoptosis. Curr Mol Med 12 (10): 1372-1382.

NCBI and Uniprot Product Information

• NCBI GI #: 25282391
• NCBI GeneID: 11270
• NCBI Accession #: NP_009174.1
• NCBI GenBank Nucleotide #: NM_007243.2
• UniProt Accession #: Q8IXM6; Q5JP57; Q5JP58; Q5JP59; Q5JP60; Q8WU45; Q9BSX3; Q9UN92; B0S7R0; B0S7R1; I3XIE2; I3XIE3
• Molecular Weight: 10,526 Da
• NCBI Official Full Name: nurim isoform 1
• NCBI Official Synonym Full Names: nurim (nuclear envelope membrane protein)
• NCBI Official Symbol: NRM
• NCBI Official Synonym Symbols: NRM29
• NCBI Protein Information: nurim; nuclear rim protein
• UniProt Protein Name: Nurim
• UniProt Gene Name: NRM
• UniProt Synonym Gene Names: NRM29
• UniProt Entry Name: NRM_HUMAN