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IP (Immunoprecipitation)
(Immunoprecipitating TIA1 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA118799 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA118799(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
Rabbit anti-Human Nucleolysin TIA-1 isoform p40 Polyclonal Antibody | anti-TIA1 antibody
Rabbit anti-human Nucleolysin TIA-1 isoform p40 polyclonal Antibody(TIA1)
Gene Names
TIA1; WDM; TIA-1
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
Nucleolysin TIA-1 isoform p40, Antibody; Rabbit anti-human Nucleolysin TIA-1 isoform p40 polyclonal Antibody(TIA1); RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA-1; p40-TIA-1; TIA1; anti-TIA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
375
Applicable Applications for anti-TIA1 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Nucleolysin TIA-1 isoform p40 protein (1-193aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1751 / sc-34739 / sc-28237
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating TIA1 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA118799 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA118799(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating TIA1 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA118799 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA118799(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human skin using AAA118799 at dilution 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human skin using AAA118799 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using AAA118799 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using AAA118799 at dilution 1:100)
WB (Western Blot)
(All lanes: Nucleolysin TIA-1 isoform p40 antibody at 2ug/mlLane 1:jurkat whole cell lysateLane 2:K562 whole cell lysateLane 3:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 43,42,25 kDaObserved band size: 43 kDa)
WB (Western Blot)
(All lanes: Nucleolysin TIA-1 isoform p40 antibody at 2ug/mlLane 1:jurkat whole cell lysateLane 2:K562 whole cell lysateLane 3:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 43,42,25 kDaObserved band size: 43 kDa)
Related Product Information for anti-TIA1 antibody
Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,062 Da
NCBI Official Full Name
nucleolysin TIA-1 isoform p40 isoform 1
NCBI Official Synonym Full Names
TIA1 cytotoxic granule-associated RNA binding protein
NCBI Official Symbol
TIA1
NCBI Official Synonym Symbols
WDM; TIA-1
NCBI Protein Information
nucleolysin TIA-1 isoform p40
UniProt Protein Name
Nucleolysin TIA-1 isoform p40
UniProt Gene Name
TIA1
UniProt Synonym Gene Names
TIA-1
UniProt Entry Name
TIA1_HUMAN
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Product Notes
The TIA1 tia1 (Catalog #AAA118799) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Nucleolysin TIA-1 isoform p40 polyclonal Antibody(TIA1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleolysin TIA-1 isoform p40 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TIA1 tia1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleolysin TIA-1 isoform p40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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