IF (Immunofluorescence)
(Fluorescent confocal image of SY5Y cells stained with AAA288564 OCT4 (E125) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AAA288564 OCT4 (E125) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). OCT4 immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.)
Rabbit anti-Human OCT4 (OCT3) Polyclonal Antibody | anti-POU5F1 antibody
OCT4 (OCT3) Antibody (E125)
Gene Names
POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
OCT4 (OCT3), Antibody; OCT4 (OCT3) Antibody (E125); POU domain; class 5; transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3; POU5F1; OCT3; OCT4; OTF3; anti-POU5F1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-141 amino acids from human OCT4 (OCT3).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
110-141
Sequence Length
360
Applicable Applications for anti-POU5F1 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of SY5Y cells stained with AAA288564 OCT4 (E125) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AAA288564 OCT4 (E125) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). OCT4 immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.)
IF (Immunofluorescence)
(Fluorescent confocal image of SY5Y cells stained with AAA288564 OCT4 (E125) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AAA288564 OCT4 (E125) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). OCT4 immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.)
WB (Western Blot)
(Western blot analysis of OCT3/4 Antibody (E125) in HL60 cell line lysates (35ug/lane). OCT3/4 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of OCT3/4 Antibody (E125) in HL60 cell line lysates (35ug/lane). OCT3/4 (arrow) was detected using the purified Pab.)
Related Product Information for anti-POU5F1 antibody
Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.
Product Categories/Family for anti-POU5F1 antibody
References
Looijenga, L.H., et al., Cancer Res. 63(9):2244-2250 (2003). Remenyi, A., et al., Genes Dev. 17(16):2048-2059 (2003). Nichols, J., et al., Cell 95(3):379-391 (1998). Crouau-Roy, B., et al., Genomics 21(1):241-243 (1994). Takeda, J., et al., Nucleic Acids Res. 20(17):4613-4620 (1992).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38571
NCBI Official Full Name
POU domain, class 5, transcription factor 1 isoform 1
NCBI Official Synonym Full Names
POU class 5 homeobox 1
NCBI Official Symbol
POU5F1
NCBI Official Synonym Symbols
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
NCBI Protein Information
POU domain, class 5, transcription factor 1
UniProt Protein Name
POU domain, class 5, transcription factor 1
UniProt Gene Name
POU5F1
UniProt Synonym Gene Names
OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
UniProt Entry Name
PO5F1_HUMAN
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Product Notes
The POU5F1 pou5f1 (Catalog #AAA288564) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 110-141. The OCT4 (OCT3) Antibody (E125) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OCT4 (OCT3) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the POU5F1 pou5f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OCT4 (OCT3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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