<
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Pan Phospho-Tyrosine antibody . Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody at a dilition of 1:1000.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.)
Rabbit Pan Phospho-Tyrosine Polyclonal Antibody
Pan Phospho-Tyrosine Rabbit pAb
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Immunoprecipitation, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
Pan Phospho-Tyrosine, Antibody; Pan Phospho-Tyrosine Rabbit pAb; anti-Pan Phospho-Tyrosine antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-Pan Phospho-Tyrosine antibody
IP (Immunoprecipitation), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence containing phosphorylated Y.
Positive Samples
Jurkat
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Pan Phospho-Tyrosine antibody . Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody at a dilition of 1:1000.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Pan Phospho-Tyrosine antibody . Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody at a dilition of 1:1000.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.)
WB (Western Blot)
(Western blot analysis of extracts of Jurkat cells, using Pan Phospho-Tyrosine pAb at 1:1000 dilution.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of Jurkat cells, using Pan Phospho-Tyrosine pAb at 1:1000 dilution.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Product Categories/Family for anti-Pan Phospho-Tyrosine antibody
Similar Products
Product Notes
The Pan Phospho-Tyrosine (Catalog #AAA282118) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pan Phospho-Tyrosine Rabbit pAb reacts with Human, Mouse, Rat, Other (Wide Range) and may cross-react with other species as described in the data sheet. AAA Biotech's Pan Phospho-Tyrosine can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Pan Phospho-Tyrosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pan Phospho-Tyrosine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.