IP (Immunoprecipitation)
(KRT was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-KRT rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-KRT rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 62 kDaObserved band size: 45 kDa)
Rabbit anti-Human Pan Cytokeratin/P-CK Polyclonal Antibody | anti-Pan antibody
Anti-Pan Cytokeratin/P-CK Antibody, Rabbit Polyclonal
Gene Names
KRT9; K9; CK-9; EPPK
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Pan Cytokeratin/P-CK, Antibody; Anti-Pan Cytokeratin/P-CK Antibody, Rabbit Polyclonal; Pan Cytokeratin/KRT(pan) Antibody, Rabbit PAb, Antigen Affinity Purified; anti-Pan antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Pan Cytokeratin/KRT (pan)
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-Pan antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Pan Cytokeratin/KRT(pan)
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Pan Cytokeratin/KRT(pan), and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(KRT was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-KRT rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-KRT rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 62 kDaObserved band size: 45 kDa)
IP (Immunoprecipitation)
(KRT was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-KRT rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-KRT rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 62 kDaObserved band size: 45 kDa)
WB (Western Blot)
(Anti-KRT(9-23) rabbit polyclonal antibody at 1:500 dilutionLane A: COLO205 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:62 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-KRT(9-23) rabbit polyclonal antibody at 1:500 dilutionLane A: COLO205 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:62 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-Pan antibody
Cytokeratins are fibrous intermediate-filament protein polymers present in almost all animal cells. There are 20 known cytokeratins, classified according to their molecular weight and pH as type I or acidic (cytokeratins 9-20) and type II or neutral-basic (cytokeratins 1-8). Cytokeratins have been identified as potential markers for the detection of circulating cancer cells by flow cytometry and RT-PCR. The pan-cytokeratin antibody has been mainly exploited in prognostic studies of circulating and disseminated epithelial tumor cells, particularly in assessing sentinel lymph node biopsies in breast cancer.
References
Rajkovic N, et al. (2018) The pan-cytokeratin staining intensity and fractal computational analysis of breast tumor malignant growth patterns prognosticate the occurrence of distant metastasis. Front Oncol 8 348.Jacques C, et al. (2005) Cytokeratins and dermatology. Skinmed 4 (6): 354-360; quiz 360-351.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62064 MW
NCBI Official Full Name
keratin, type I cytoskeletal 9
NCBI Official Synonym Full Names
keratin 9
NCBI Official Symbol
KRT9
NCBI Official Synonym Symbols
K9; CK-9; EPPK
NCBI Protein Information
keratin, type I cytoskeletal 9
UniProt Protein Name
Keratin, type I cytoskeletal 9
UniProt Gene Name
KRT9
UniProt Synonym Gene Names
CK-9; K9
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Product Notes
The Pan krt9 (Catalog #AAA254304) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Pan Cytokeratin/P-CK Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pan Cytokeratin/P-CK can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Pan krt9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pan Cytokeratin/P-CK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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