WB (Western Blot)
(Detection of human PAPD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-PAPD1 antibody AAA212363 (lot AAA212363-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human PAPD1 Polyclonal Antibody | anti-PAPD1 antibody
Rabbit anti-PAPD1 Antibody, Affinity Purified
Gene Names
MTPAP; PAPD1; SPAX4
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PAPD1, Antibody; Rabbit anti-PAPD1 Antibody, Affinity Purified; terminal uridylyltransferase 1; TUTase 1; TENT6; SPAX4; polynucleotide adenylyltransferase; PAPD1; PAP-associated domain-containing protein 1; PAP; mtPAP; poly(A) RNA polymerase, mitochondrial; Poly(A) RNA polymerase, mitochondrial; mitochondrial poly(A) polymerase; anti-PAPD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PAPD1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 532 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PAPD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-PAPD1 antibody AAA212363 (lot AAA212363-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PAPD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-PAPD1 antibody AAA212363 (lot AAA212363-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PAPD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PAPD1 antibody AAA212363 (lot AAA212363-1) used for IP at 6 ug/mg lysate. PAPD1 was also immunoprecipitated by rabbit anti-PAPD1 antibody BL14103. For blotting immunoprecipitated PAPD1, AAA212363 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PAPD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PAPD1 antibody AAA212363 (lot AAA212363-1) used for IP at 6 ug/mg lysate. PAPD1 was also immunoprecipitated by rabbit anti-PAPD1 antibody BL14103. For blotting immunoprecipitated PAPD1, AAA212363 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-PAPD1 antibody
PAPD1 (mitochondrial poly(A) polymerase) is a polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. PAPD1 can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). It plays a role in replication-dependent histone mRNA degradation. It may be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated and might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA [taken from NCBI Entrez Gene (Gene ID: 55149)].
Product Categories/Family for anti-PAPD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78,833 Da
NCBI Official Full Name
poly(A) RNA polymerase, mitochondrial
NCBI Official Synonym Full Names
mitochondrial poly(A) polymerase
NCBI Official Symbol
MTPAP
NCBI Official Synonym Symbols
PAPD1; SPAX4
NCBI Protein Information
poly(A) RNA polymerase, mitochondrial; poly(A) RNA polymerase, mitochondrial; PAP-associated domain-containing protein 1; TUTase 1; polynucleotide adenylyltransferase; terminal uridylyltransferase 1
UniProt Protein Name
Poly(A) RNA polymerase, mitochondrial
UniProt Gene Name
MTPAP
UniProt Synonym Gene Names
PAPD1; PAP; TUTase 1
UniProt Entry Name
PAPD1_HUMAN
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Product Notes
The PAPD1 mtpap (Catalog #AAA212363) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PAPD1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAPD1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PAPD1 mtpap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAPD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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