IF (Immunofluorescence)
(Immunofluorescent analysis of Parkin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30.)
Rabbit Parkin Polyclonal Antibody | anti-PRKN antibody
Parkin Antibody
Gene Names
PRKN; PDJ; AR-JP; LPRS2; PARK2
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Immunogen Affinity Chromatography
Synonyms
Parkin, Antibody; Parkin Antibody; Purified Polyclonal Parkin Antibody; Polyclonal Parkin antibody; Anti-Parkin antibody; PRKN antibody; E3 ubiquitin-protein ligase parkin antibody; Parkinson juvenile disease protein 2 antibody; Parkinson disease protein 2 antibody; anti-PRKN antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Parkin protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Concentration
1.0 mg/mL (varies by lot)
Sequence Length
465
Applicable Applications for anti-PRKN antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Parkin. The exact sequence is proprietary .
Cross-Reactivity
Human, Rat, Bovine, Monkey, Pig
Preparation and Storage
Upon receipt store at -20°C. Avoid repeat freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of Parkin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30.)
IF (Immunofluorescence)
(Immunofluorescent analysis of Parkin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30.)
WB (Western Blot)
(Western blot analysis of Parkin expression in HepG2 (A); SHSY5Y (B); SW480 (C) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of Parkin expression in HepG2 (A); SHSY5Y (B); SW480 (C) whole cell lysates.)
Related Product Information for anti-PRKN antibody
The precise function of this protein is unknown; however, the protein is a component of a multiprotein E3 ubiquitin ligase complex which in turn is part of the ubiquitin-proteasome system that mediates the targeting of proteins for degradation.
Product Categories/Family for anti-PRKN antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PRKN (Catalog #AAA226247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Parkin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the PRKN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Parkin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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