<
WB (Western Blot)
(Detection of human PARN by western blot. Samples: Whole cell lysate (10 ug) from LNCaP, MCF-7, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (AAA212226 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse PARN Polyclonal Antibody | anti-PARN antibody
Rabbit anti-PARN Antibody, Affinity Purified
Gene Names
PARN; DAN
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PARN, Antibody; Rabbit anti-PARN Antibody, Affinity Purified; deadenylation nuclease; polyadenylate-specific ribonuclease; poly(A)-specific ribonuclease PARN; DKCB6; deadenylating nuclease; DAN; PFBMFT4; Poly(A)-specific ribonuclease PARN; poly(A)-specific ribonuclease; anti-PARN antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PARN antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 589 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PARN by western blot. Samples: Whole cell lysate (10 ug) from LNCaP, MCF-7, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (AAA212226 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PARN by western blot. Samples: Whole cell lysate (10 ug) from LNCaP, MCF-7, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (AAA212226 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse PARN by western blot. Samples: Whole cell lysate (10 ug) from CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (AAA212226 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse PARN by western blot. Samples: Whole cell lysate (10 ug) from CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (AAA212226 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PARN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARN antibody (AAA212226 lot 2) used for IP at 6 ug per reaction. PARN was also immunoprecipitated by a previous lot of this antibody (AAA212226 lot 1) and a second antibody against a different epitope of PARN . For blotting immunoprecipitated PARN, AAA212226 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human PARN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARN antibody (AAA212226 lot 2) used for IP at 6 ug per reaction. PARN was also immunoprecipitated by a previous lot of this antibody (AAA212226 lot 1) and a second antibody against a different epitope of PARN . For blotting immunoprecipitated PARN, AAA212226 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human PARN by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PARN (AAA212226 Lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human PARN by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PARN (AAA212226 Lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
Related Product Information for anti-PARN antibody
Poly(A)-specific ribonuclease (PARN) is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. PARN prefers poly(A) as the substrate, hence, it efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. PARN is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons [taken from NCBI Entrez Gene (Gene ID: 5073)].
Product Categories/Family for anti-PARN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,722 Da
NCBI Official Full Name
poly(A)-specific ribonuclease PARN isoform 1
NCBI Official Synonym Full Names
poly(A)-specific ribonuclease
NCBI Official Symbol
PARN
NCBI Official Synonym Symbols
DAN
NCBI Protein Information
poly(A)-specific ribonuclease PARN; poly(A)-specific ribonuclease PARN; deadenylating nuclease; deadenylation nuclease; polyadenylate-specific ribonuclease; poly(A)-specific ribonuclease (deadenylation nuclease)
UniProt Protein Name
Poly(A)-specific ribonuclease PARN
UniProt Gene Name
PARN
UniProt Synonym Gene Names
DAN
UniProt Entry Name
PARN_HUMAN
Similar Products
Product Notes
The PARN parn (Catalog #AAA212226) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PARN Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PARN can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PARN parn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.