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IP (Immunoprecipitation)
(PARP1 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 µL anti-PARP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PARP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 113 kDaObserved band size :120 kDa)
Rabbit anti-Human PARP-1 Polyclonal Antibody | anti-PARP-1 antibody
Anti-PARP-1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
PARP-1, Antibody; Anti-PARP-1 Antibody, Rabbit Polyclonal; poly (ADP-ribose) polymerase 1; Anti-ADPRT Antibody; Anti-ADPRT1 Antibody; Anti-ARTD1 Antibody; Anti-pADPRT-1 Antibody; Anti-PARP Antibody; Anti-PARP-1 Antibody; Anti-PPOL Antibody; anti-PARP-1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PARP-1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PARP-1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:1000-1:5000
ICC/IF: 1:100-1:500
IP: 1-5uL/mg of lysate
IHC-P: 1:1000-1:5000
ICC/IF: 1:100-1:500
IP: 1-5uL/mg of lysate
Immunogen
E coli-derived Human PARP fragment
Preparation
Produced in rabbits immunized with E coli-derived Human PARP fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PARP1 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 µL anti-PARP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PARP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 113 kDaObserved band size :120 kDa)
IP (Immunoprecipitation)
(PARP1 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 µL anti-PARP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PARP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 113 kDaObserved band size :120 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of PARP1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PARP1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of PARP1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PARP1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of human PARP1 in human lymph node with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of human PARP1 in human lymph node with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of human PARP1 in human placenta with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of human PARP1 in human placenta with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of human PARP1 in human prostate with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining of human PARP1 in human prostate with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-PARP1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela(1um Staurosporine treatment for 3h) Whole Cell LysateLane B: Hela Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:113 kDaObserved band size:113 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-PARP1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela(1um Staurosporine treatment for 3h) Whole Cell LysateLane B: Hela Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:113 kDaObserved band size:113 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-PARP-1 antibody
Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrated to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors are thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.
References
Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843.
Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625.
Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178.
Ahell I. et al., 2008, Nature. 451: 81-85.
Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625.
Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178.
Ahell I. et al., 2008, Nature. 451: 81-85.
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Product Notes
The PARP-1 (Catalog #AAA27781) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PARP-1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:1000-1:5000 ICC/IF: 1:100-1:500 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the PARP-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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