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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PAX6-T373 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human PAX6-T373 Polyclonal Antibody | anti-PAX6 antibody
PAX6-T373 Antibody
Gene Names
PAX6; AN; AN2; FVH1; MGDA; WAGR; D11S812E
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PAX6-T373, Antibody; PAX6-T373 Antibody; Paired box protein Pax-6; Aniridia type II protein; Oculorhombin; PAX6; AN2; anti-PAX6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-380 amino acids from human PAX6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.25 (varies by lot)
Sequence Positions
352-380
Sequence Length
422
Applicable Applications for anti-PAX6 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PAX6-T373 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PAX6-T373 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemisry)
(PAX6-T373 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAX6-T373 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(PAX6-T373 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAX6-T373 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of PAX6 (arrow) using rabbit polyclonal PAX6 Antibody (T373). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.)
WB (Western Blot)
(Western blot analysis of PAX6 (arrow) using rabbit polyclonal PAX6 Antibody (T373). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.)
WB (Western Blot)
(Western blot analysis of PAX6-T373 in Y79 cell line lysates (35ug/lane). PAX6 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of PAX6-T373 in Y79 cell line lysates (35ug/lane). PAX6 (arrow) was detected using the purified Pab.)
Related Product Information for anti-PAX6 antibody
PAX6 encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly.
Product Categories/Family for anti-PAX6 antibody
References
Zhang, Y., et al. J. Biol. Chem. 285(4):2527-2536(2010)
McGeachie, M., et al. Circulation 120(24):2448-2454(2009)
Schmidt-Sidor, B., et al. Folia Neuropathol 47(4):372-382(2009)
Ng, T.K., et al. Mol. Vis. 15, 2239-2248 (2009)
McGeachie, M., et al. Circulation 120(24):2448-2454(2009)
Schmidt-Sidor, B., et al. Folia Neuropathol 47(4):372-382(2009)
Ng, T.K., et al. Mol. Vis. 15, 2239-2248 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46683
NCBI Official Full Name
paired box protein Pax-6 isoform a
NCBI Official Synonym Full Names
paired box 6
NCBI Official Symbol
PAX6
NCBI Official Synonym Symbols
AN; AN2; FVH1; MGDA; WAGR; D11S812E
NCBI Protein Information
paired box protein Pax-6
UniProt Protein Name
Paired box protein Pax-6
UniProt Gene Name
PAX6
UniProt Synonym Gene Names
AN2
UniProt Entry Name
PAX6_HUMAN
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Product Notes
The PAX6 pax6 (Catalog #AAA289216) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 352-380. The PAX6-T373 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAX6-T373 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PAX6 pax6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAX6-T373, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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