WB (Western Blot)
(Detection of human PC4 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PC4 antibody AAA211340 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B). PC4 was immunoprecipitated by rabbit anti-PC4 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
Rabbit anti-Human, Mouse PC4 Polyclonal Antibody | anti-PC4 antibody
Rabbit anti-PC4 Antibody, Affinity Purified
Gene Names
SUB1; P15; PC4; p14
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PC4, Antibody; Rabbit anti-PC4 Antibody, Affinity Purified; activated RNA polymerase II transcriptional coactivator p15; SUB1 homolog; positive cofactor 4; PC4; p14; activated RNA polymerase II transcription cofactor 4; P15; Activated RNA polymerase II transcriptional coactivator p15; SUB1 homolog, transcriptional regulator; anti-PC4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PC4 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Predicted Reactivity
Rat, Chicken, Orangutan
Immunogen
Between 77 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PC4 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PC4 antibody AAA211340 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B). PC4 was immunoprecipitated by rabbit anti-PC4 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
WB (Western Blot)
(Detection of human PC4 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PC4 antibody AAA211340 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B). PC4 was immunoprecipitated by rabbit anti-PC4 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
IHC (Immunohistochemistry)
(Detection of human and mouse PC4 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PC4 (Cat. No. AAA211340 Lot1) used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human and mouse PC4 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PC4 (Cat. No. AAA211340 Lot1) used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-PC4 antibody
PC4 is a multifunctional transcriptional coactivator that plays an important role in transcription, repair, and replication. PC4 interacts with general transcription factors, DNA repair factors, and mRNA processing factors to influence transcription initiation, coactivation, polyadenylation, transcription termination, and transcriptional repression. PC4 has been demonstrated to be a DNA binding protein and a bona fide component of chromatin with chromatin-compacting ability.
Product Categories/Family for anti-PC4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,395 Da
NCBI Official Full Name
activated RNA polymerase II transcriptional coactivator p15
NCBI Official Synonym Full Names
SUB1 homolog (S. cerevisiae)
NCBI Official Symbol
SUB1
NCBI Official Synonym Symbols
P15; PC4; p14
NCBI Protein Information
activated RNA polymerase II transcriptional coactivator p15; activated RNA polymerase II transcription cofactor 4; positive cofactor 4
UniProt Protein Name
Activated RNA polymerase II transcriptional coactivator p15
UniProt Gene Name
SUB1
UniProt Synonym Gene Names
PC4; RPO2TC1; PC4
UniProt Entry Name
TCP4_HUMAN
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Product Notes
The PC4 sub1 (Catalog #AAA211340) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PC4 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PC4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PC4 sub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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