Rabbit PC4/SUB1 Polyclonal Antibody | anti-SUB1 antibody

Anti-PC4/SUB1 Antibody Picoband

Cat. Num. AAA126243

• Gene Names: SUB1; P15; PC4; p14
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: PC4/SUB1, Antibody; Anti-PC4/SUB1 Antibody Picoband; anti-SUB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)

• Applicable Applications for anti-SUB1 antibody: ELISA, IHC (Immunohistochemistry), WB (Western Blot)
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
• Immunogen: E Coli-derived human PC4/SUB1 recombinant protein (Position: N62-L127).
• Preparation and Storage: Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

IHC (Immunohistochemistry)

(Figure 5. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA126243).PC4/SUB1 was detected in a paraffin-embedded section of human papillary carcinoma of the left breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PC4/SUB1 Antibody (AAA126243) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA126243).PC4/SUB1 was detected in a paraffin-embedded section of human gastric signet ring cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PC4/SUB1 Antibody (AAA126243) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemisry)

(Figure 3. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA126243).PC4/SUB1 was detected in a paraffin-embedded section of human gastric signet ring cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PC4/SUB1 Antibody (AAA126243) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry)

(Figure 2. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA126243).PC4/SUB1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PC4/SUB1 Antibody (AAA126243) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA126243).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human T-47D whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: rat pancreas tissue lysates,Lane 6: mouse pancreas tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC4/SUB1 antigen affinity purified polyclonal antibody (#AAA126243) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 19 kDa. The expected band size for PC4/SUB1 is at 14 kDa.)

Related Product Information for anti-SUB1 antibody

Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA)-binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs.

Product Categories/Family for anti-SUB1 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 1709514
• NCBI GeneID: 10923
• UniProt Accession #: P53999; Q96L29
• Molecular Weight: 127
• NCBI Official Full Name: Activated RNA polymerase II transcriptional coactivator p15
• NCBI Official Synonym Full Names: SUB1 homolog (S. cerevisiae)
• NCBI Official Symbol: SUB1
• NCBI Official Synonym Symbols: P15; PC4; p14
• NCBI Protein Information: activated RNA polymerase II transcriptional coactivator p15; positive cofactor 4; activated RNA polymerase II transcription cofactor 4
• UniProt Protein Name: Activated RNA polymerase II transcriptional coactivator p15
• UniProt Gene Name: SUB1
• UniProt Synonym Gene Names: PC4; RPO2TC1; PC4
• UniProt Entry Name: TCP4_HUMAN