IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PCDHGA8 Antibody (C-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human PCDHGA8 Polyclonal Antibody | anti-PCDHGA8 antibody
PCDHGA8 Antibody (C-term)
Gene Names
PCDHGA8; PCDH-GAMMA-A8
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PCDHGA8, Antibody; PCDHGA8 Antibody (C-term); Protocadherin gamma-A8; PCDH-gamma-A8; PCDHGA8; KIAA0327; anti-PCDHGA8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PCDHGA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 781-808 amino acids from the C-terminal region of human PCDHGA8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.32 (varies by lot)
Sequence Positions
781-808
Sequence Length
820
Applicable Applications for anti-PCDHGA8 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PCDHGA8 Antibody (C-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PCDHGA8 Antibody (C-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(PCDHGA8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHGA8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PCDHGA8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHGA8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PCDHGA8 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCDHGA8 antibody detected the PCDHGA8 protein (arrow).)
WB (Western Blot)
(PCDHGA8 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCDHGA8 antibody detected the PCDHGA8 protein (arrow).)
Related Product Information for anti-PCDHGA8 antibody
This gene is a member of the protocadherin gamma gene
cluster, one of three related clusters tandemly linked on
chromosome five. These gene clusters have an immunoglobulin-like
organization, suggesting that a novel mechanism may be involved in
their regulation and expression. The gamma gene cluster includes 22
genes divided into 3 subfamilies. Subfamily A contains 12 genes,
subfamily B contains 7 genes and 2 pseudogenes, and the more
distantly related subfamily C contains 3 genes. The tandem array of
22 large, variable region exons are followed by a constant region,
containing 3 exons shared by all genes in the cluster. Each
variable region exon encodes the extracellular region, which
includes 6 cadherin ectodomains and a transmembrane region. The
constant region exons encode the common cytoplasmic region. These
neural cadherin-like cell adhesion proteins most likely play a
critical role in the establishment and function of specific
cell-cell connections in the brain. Alternative splicing has been
described for the gamma cluster genes.
cluster, one of three related clusters tandemly linked on
chromosome five. These gene clusters have an immunoglobulin-like
organization, suggesting that a novel mechanism may be involved in
their regulation and expression. The gamma gene cluster includes 22
genes divided into 3 subfamilies. Subfamily A contains 12 genes,
subfamily B contains 7 genes and 2 pseudogenes, and the more
distantly related subfamily C contains 3 genes. The tandem array of
22 large, variable region exons are followed by a constant region,
containing 3 exons shared by all genes in the cluster. Each
variable region exon encodes the extracellular region, which
includes 6 cadherin ectodomains and a transmembrane region. The
constant region exons encode the common cytoplasmic region. These
neural cadherin-like cell adhesion proteins most likely play a
critical role in the establishment and function of specific
cell-cell connections in the brain. Alternative splicing has been
described for the gamma cluster genes.
Product Categories/Family for anti-PCDHGA8 antibody
References
Wu, Q., et al. Genome Res. 11(3):389-404(2001)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)
Wu, Q., et al. Cell 97(6):779-790(1999)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)
Wu, Q., et al. Cell 97(6):779-790(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101480
NCBI Official Full Name
protocadherin gamma-A8 isoform 2
NCBI Official Synonym Full Names
protocadherin gamma subfamily A, 8
NCBI Official Symbol
PCDHGA8
NCBI Official Synonym Symbols
PCDH-GAMMA-A8
NCBI Protein Information
protocadherin gamma-A8
UniProt Protein Name
Protocadherin gamma-A8
UniProt Gene Name
PCDHGA8
UniProt Synonym Gene Names
KIAA0327; PCDH-gamma-A8
UniProt Entry Name
PCDG8_HUMAN
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Product Notes
The PCDHGA8 pcdhga8 (Catalog #AAA289896) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 781-808. The PCDHGA8 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCDHGA8 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PCDHGA8 pcdhga8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCDHGA8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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