Rabbit anti-Human PDCD10 Polyclonal Antibody | anti-PDCD10 antibody

PDCD10 Antibody

Cat. Num. AAA301907

• Gene Names: PDCD10; CCM3; TFAR15
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Synonyms: PDCD10, Antibody; PDCD10 Antibody; programmed cell death 10, CCM3, TFAR15; anti-PDCD10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PDCD10 protein.
• Form/Format: Supplied at 0.6mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 1466

• Applicable Applications for anti-PDCD10 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen Type: Recombinant protein
• Immunogen Description: Full length fusion protein
• Predicted MW: 25kD
• Target Name: PDCD10
• IHC-P PROTOCOL: 1.Deparaffinize the section in 3 changes of xylene, 5 minutes each.; 2.Wash the section in 96%, 80% and 0% thyl alcohol for 10 minutes each. .; 3.Rinse in distilled water. .; 4.Block the endogenous peroxidase by incubating the tissuein 3% hydrogen peroxide (H2O2) for 10 minutes. .; 5.Wash in distilled water for 5 minutes. .; 6.For antigen retrieval: immerse the slide in the citrate buffer, pH 6.0, 0.05% Tween-20*, and incubate in ater bath at 96°C for 40 minutes. (Alternatively adjust to your own protocol, keeping the required pH) .; 7.Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 20 minutes. .; 8.Rinse in distilled water. .; 9.Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.; 10.CONCENTRATED:Incubate the section with primary antibody at the dilution 1:100 -1:200 for 1 hour in the closed wet chamber. .; .; READY TO USE (RTU): .; Incubate the section with primary antibody (ready to use)for 1 hour in a closed wet chamber. 11.Wash twice 5 minutes with buffer A. .; ; 12.Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP -Peroxide -DAB). Micropolymer-HRP detection kit rabbit/mouse .; 13.Wash twice 5 minutes with buffer A. .; ; 14.Apply the chromogen (DAB), 1 -3 minutes. .; ; 15.Wash in water for 10 minutes. .; 16.Stain in hematoxylin for 5 minutes. .; 17.Wash in water for 10 minutes. .; 18.Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.; 19.Wash the section in 2 changes of xylene for 2 minutes each. .; 20.Mount the slide for observation.
• Preparation and Storage: Store at -20°C/ 1 year

IHC (Immunohiostchemistry)

(The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using 31110(PDCD10 Antibody) at dilution 1/20, on the right is treated with the fusion protein.)

Application Data

(Gel: 12%SDS-PAGELane1: Hela cell lysateLane2: Jurkat cell lysateLane3: 231 cell lysateLysates: 50 ug per lanePrimary antibody: 1/200dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 10 seconds)

Related Product Information for anti-PDCD10 antibody

This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product Categories/Family for anti-PDCD10 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 12803370
• NCBI GeneID: 11235
• NCBI Official Full Name: Homo sapiens programmed cell death 10, mRNA
• NCBI Official Synonym Full Names: programmed cell death 10
• NCBI Official Symbol: PDCD10
• NCBI Official Synonym Symbols: CCM3; TFAR15
• NCBI Protein Information: programmed cell death protein 10
• UniProt Protein Name: Programmed cell death protein 10
• UniProt Gene Name: PDCD10
• UniProt Synonym Gene Names: CCM3; TFAR15
• UniProt Entry Name: PDC10_HUMAN