Rabbit anti-Human Perk Polyclonal Antibody | anti-Perk antibody

Perk Antibody

Cat. Num. AAA328610

• Gene Names: EIF2AK3; PEK; WRS; PERK
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Peptide affinity purification
• Synonyms: Perk, Antibody; Perk Antibody; DKFZp781H1925; E2AK3_HUMAN; EC 2.7.11.1; Eif2ak3; Eukaryotic translation initiation factor 2 alpha kinase 3; Eukaryotic translation initiation factor 2-alpha kinase 3; Heme regulated EIF2 alpha kinase; HRI; HsPEK; Pancreatic eIF2 alpha kinase; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; PRKR-like endoplasmic reticulum kinase; WRS; anti-Perk antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PERK Antibody detects endogenous levels of PERK.
• Purity/Purification: Peptide affinity purification
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1116

• Applicable Applications for anti-Perk antibody: ELISA (Peptide ELISA), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human perk, corresponding to a region within the internal amino acids.
• Important: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of perk in lysates of HepG2 , using perk Antibody.)

Related Product Information for anti-Perk antibody

Description: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Post Translational Modifications: Oligomerization of the N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr-982 on the kinase activation loop (By similarity). Autophosphorylated. Phosphorylated at Tyr-619 following endoplasmic reticulum stress, leading to activate its tyrosine-protein kinase activity. Dephosphorylated by PTPN1/TP1B, leading to inactivate its enzyme activity. N-glycosylated. ADP-ribosylated by PARP16 upon ER stress, which increases kinase activity.

Subunit Structure: Forms dimers with HSPA5/BIP in resting cells (By similarity). Oligomerizes in ER-stressed cells (By similarity). Interacts with DNAJC3 and MFN2 (By similarity). Interacts with TMEM33 (PubMed:26268696). Interacts with PDIA6 (PubMed:24508390).

Similarity: The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 134304838
• NCBI GeneID: 9451
• NCBI Accession #: NP_004827.4
• NCBI GenBank Nucleotide #: NP_004827.4
• UniProt Accession #: Q9NZJ5
• Molecular Weight: 125kDa
• NCBI Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2 alpha kinase 3
• NCBI Official Symbol: EIF2AK3
• NCBI Official Synonym Symbols: PEK; WRS; PERK
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 3
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 3
• UniProt Gene Name: EIF2AK3
• UniProt Synonym Gene Names: PEK; PERK; HsPEK
• UniProt Entry Name: E2AK3_HUMAN