Rabbit PEX10 Polyclonal Antibody | anti-PEX10 antibody

PEX10 (CT) Antibody

Cat. Num. AAA11049

• Gene Names: PEX10; NALD; PBD6A; PBD6B; RNF69
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: PEX10 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PEX10, Antibody; PEX10 (CT) Antibody; PEX10 Antibody: Peroxin-10; Peroxisomal biogenesis factor 10; Peroxisome assembly protein 10; RING finger protein 69; RNF69; anti-PEX10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PEX10 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; PEX10 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-PEX10 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 4ug/mL; IF: 20ug/mL; Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
• Immunogen: Anti-PEX10 antibody (9671) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10.; The immunogen is located within last 50 amino acids of PEX10.
• Homology: Predicted species reactivity based on immunogen sequence: monkey (100%), horse (100%), gorilla (100%), chimpanzee (100%)
• Research Area: Signal Transduction
• NCBI Organism: Homo sapiens
• Isoforms: Human PEX10 has 2 isoforms, including isoform 1 (326aa, 37.1kD) and isoform 2 (346aa, 39.2kD). Mouse PEX10 has 1 isoform (324aa, 37.2kD). can detect PEX10 in human and mouse.
• Preparation and Storage: PEX10 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Figure 7 Immunofluorescence Validation of PEX10 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 6 Immunofluorescence Validation of PEX10 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 5 Immunofluorescence Validation of PEX10 in Human Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 4 Immunofluorescence Validation of PEX10 in Human Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

WB (Western Blot)

(Figure 3 WB Validation in Rat TissuesLoading: 15ug of lysates per lane.Antibodies: PEX10 AAA11049, 4ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot)

(Figure 2 WB Validation in Mouse Tissues Loading: 15ug of lysates per lane.Antibodies: PEX10 AAA11049, 4ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot)

(Figure 1 WB Validation in Human and Mouse Cell LinesLoading: 15ug of lysate Antibodies: PEX10 AAA11049, 4ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)

Related Product Information for anti-PEX10 antibody

PEX10 is implicated in the biogenesis of peroxisomes. Mutations in this gene result in phenotypes within the Zellweger spectrum of peroxisomal biogenesis disorders, ranging from neonatal adrenoleukodystrophy to Zellweger syndrome.

Product Categories/Family for anti-PEX10 antibody

Primary Antibodies

References

Warren D.S. et al. Am. J. Hum. Genet. 63:347-359(1998)
Okumoto K. et al. Hum. Mol. Genet. 7:1399-1405(1998)
Ota T. et al. Nat. Genet. 36:40-45(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 3914299
• NCBI GeneID: 5192
• NCBI Accession #: NP_002608.1
• NCBI GenBank Nucleotide #: NM_002617.3
• UniProt Accession #: O60683; Q5T095; Q9BW90; B3KWD8
• Molecular Weight: 37,069 Da
• NCBI Official Full Name: peroxisome biogenesis factor 10 isoform 2
• NCBI Official Synonym Full Names: peroxisomal biogenesis factor 10
• NCBI Official Symbol: PEX10
• NCBI Official Synonym Symbols: NALD; PBD6A; PBD6B; RNF69
• NCBI Protein Information: peroxisome biogenesis factor 10; peroxin 10; peroxin-10; RING finger protein 69; peroxisome assembly protein 10
• UniProt Protein Name: Peroxisome biogenesis factor 10
• UniProt Gene Name: PEX10
• UniProt Synonym Gene Names: RNF69
• UniProt Entry Name: PEX10_HUMAN