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IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of PGAM5 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with AAA11041 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Rabbit PGAM5 Polyclonal Antibody | anti-PGAM5 antibody
PGAM5 (CT) Antibody
Gene Names
PGAM5; BXLBV68
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunofluorescence
Purity
PGAM5 Antibody is affinity chromatography purified via peptide column.
Synonyms
PGAM5; Polyclonal Antibody; PGAM5 (CT) Antibody; Serine/threonine-protein phosphatase PGAM5 mitochondrial; Bcl-XL-binding protein v68; Phosphoglycerate mutase family member 5; PGAM family member 5; mitochondrial serine/threonine protein phosphatase; anti-PGAM5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
PGAM5 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; PGAM5 Antibody is supplied in PBS containing 0.02% sodium azide
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-PGAM5 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 0.5ug/mL; IF: 10-20ug/mL
Antibody validated: Western Blot in human, mouse, and rat samples; Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
Immunogen
Anti-PGAM5 antibody (9517) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5.
The immunogen is located within the last 50 amino acids of PGAM5.
The immunogen is located within the last 50 amino acids of PGAM5.
Homology
Predicted species reactivity based on immunogen sequence: gorilla (100%); Chimpanzee (100%); monkey (100%).
Research Area
Signal Transduction
NCBI Organism
Homo sapiens
Isoforms
Human PGAM5 has 2 isoforms, including isoform 1 (289aa, 32kD), and isoform 2 (255aa, 28kD). Mouse PGAM5 has 2 isoforms, including isoform 1 (288aa, 32kD) and isoform 2 (287aa, 31.9kD). Rat PGAM5 has one isoform (288aa, 32kD). can detect PGAM5 in human, mouse and rat.
Preparation and Storage
PGAM5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of PGAM5 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with AAA11041 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of PGAM5 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with AAA11041 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of PGAM5 in Mouse TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with AAA11041 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of PGAM5 in Mouse TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with AAA11041 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of PGAM5 in Human LiverImmunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with AAA11041 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of PGAM5 in Human LiverImmunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with AAA11041 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Figure 4 Western Blot Validation in Rat Tissues Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 4 Western Blot Validation in Rat Tissues Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 3 Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 3 Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2 Western Blot Validation in Human Cell Lines Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2 Western Blot Validation in Human Cell Lines Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1 Western Blot Validation in Mouse and Rat Cell Lines Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1 Western Blot Validation in Mouse and Rat Cell Lines Loading: 15ug of lysates per lane. Antibodies: PGAM5, AAA11041 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
Related Product Information for anti-PGAM5 antibody
PGAM5 Antibody: PGAM5 is a central mediator for programmed necrosis induced by TNF and may be a regulator of mitochondrial dynamics. It displays phosphatase activity for serine/threonine residues, dephosphorylates and activates MAP3K5 kinase. It has apparently no phosphoglycerate mutase activity and is a substrate for a KEAP1-dependent ubiquitin ligase complex. It is also contributes to the repression of NFE2L2-dependent gene expression
Product Categories/Family for anti-PGAM5 antibody
References
Lo S. et al. Exp. Cell Res. 2018; 314(8): 1789-1803
Takeda et al. PNAS 2009; 106(30): 12301-12305
Want et al. Cell 2012; 148(1-2); 228-243
Takeda et al. PNAS 2009; 106(30): 12301-12305
Want et al. Cell 2012; 148(1-2); 228-243
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,004 Da
NCBI Official Full Name
serine/threonine-protein phosphatase PGAM5, mitochondrial isoform 1
NCBI Official Synonym Full Names
phosphoglycerate mutase family member 5
NCBI Official Symbol
PGAM5
NCBI Official Synonym Symbols
BXLBV68
NCBI Protein Information
serine/threonine-protein phosphatase PGAM5, mitochondrial; Bcl-XL-binding protein v68
UniProt Protein Name
Serine/threonine-protein phosphatase PGAM5, mitochondrial
UniProt Gene Name
PGAM5
UniProt Entry Name
PGAM5_HUMAN
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Product Notes
The PGAM5 pgam5 (Catalog #AAA11041) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGAM5 (CT) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PGAM5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 0.5ug/mL; IF: 10-20ug/mL Antibody validated: Western Blot in human, mouse, and rat samples; Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the PGAM5 pgam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGAM5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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