IHC (Immunohiostchemistry)
(Anti-PGK1 antibody, AAA45402, IHC(P)IHC(P): Human Lung Cancer Tissue)
Rabbit PGK1 Polyclonal Antibody | anti-PGK1 antibody
Anti-PGK1 antibody
Gene Names
PGK1; PGKA; MIG10; HEL-S-68p
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
PGK1, Antibody; Anti-PGK1 antibody; Phosphoglycerate kinase 1; phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein antibody; MGC117307 antibody; MGC8947 antibody; MIG10 antibody; pgk1 antibody; PGK1_HUMAN antibody; PGKA antibody; Phosphoglycerate kinase 1 antibody; Primer recognition protein 2 antibody; PRP 2 antibody; anti-PGK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
417
Applicable Applications for anti-PGK1 antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PGK1 (166-180aa FGTAHRAHSSMVGVN), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-PGK1 antibody, AAA45402, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-PGK1 antibody, AAA45402, IHC(P)IHC(P): Human Lung Cancer Tissue)
WB (Western Blot)
(Anti-PGK1 antibody, AAA45402, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Lung Tissue LysateLane 4: A431 Cell LysateLane 5: COLO320 Cell LysateLane 6: HELA Cell LysateLane 7: A549 Cell LysateLane 8: JURKAT Cell Lysate)
WB (Western Blot)
(Anti-PGK1 antibody, AAA45402, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Lung Tissue LysateLane 4: A431 Cell LysateLane 5: COLO320 Cell LysateLane 6: HELA Cell LysateLane 7: A549 Cell LysateLane 8: JURKAT Cell Lysate)
Related Product Information for anti-PGK1 antibody
Description: Rabbit IgG polyclonal antibody for Phosphoglycerate kinase 1(PGK1) detection. Tested with WB in Human, Mouse, Rat.
Background: PGK1(Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. Willard et al.(1985) used a cDNA for human PGK to map the functional PGK1 gene to Xq13. Disulfide bonds in secreted proteins are considered to be inert because of the oxidizing nature of the extracellular milieu. An exception to this rule is a reductase secreted by tumor cells that reduces disulfide bonds in the serine proteinase plasmin. Lay et al.(2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. Recombinant phosphoglycerate kinase had the same specific activity as the fibrosarcoma-derived protein. Plasma of mice bearing fibrosarcoma tumors contained several-fold more phosphoglycerate kinase, as compared with mice without tumors.
Background: PGK1(Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. Willard et al.(1985) used a cDNA for human PGK to map the functional PGK1 gene to Xq13. Disulfide bonds in secreted proteins are considered to be inert because of the oxidizing nature of the extracellular milieu. An exception to this rule is a reductase secreted by tumor cells that reduces disulfide bonds in the serine proteinase plasmin. Lay et al.(2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. Recombinant phosphoglycerate kinase had the same specific activity as the fibrosarcoma-derived protein. Plasma of mice bearing fibrosarcoma tumors contained several-fold more phosphoglycerate kinase, as compared with mice without tumors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,429 Da
NCBI Official Full Name
Phosphoglycerate kinase 1
NCBI Official Synonym Full Names
phosphoglycerate kinase 1
NCBI Official Symbol
PGK1
NCBI Official Synonym Symbols
PGKA; MIG10; HEL-S-68p
NCBI Protein Information
phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p
UniProt Protein Name
Phosphoglycerate kinase 1
UniProt Gene Name
PGK1
UniProt Synonym Gene Names
PGKA; PRP 2
UniProt Entry Name
PGK1_HUMAN
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Product Notes
The PGK1 pgk1 (Catalog #AAA45402) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PGK1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PGK1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PGK1 pgk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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