WB (Western Blot)
(Western blot analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, AAA76716, dilution 1:2,000 in green, and mouse mAb to Actin in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties.)
Chicken PGP9.5/UCHL1 Polyclonal Antibody | anti-PGP9.5/UCHL1 antibody
PGP9.5/UCHL1
Gene Names
UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
Reactivity
Human, Mouse, Rat, Bovine, Porcine, Equine
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
Synonyms
PGP9.5/UCHL1, Antibody; PGP9.5/UCHL1; anti-PGP9.5/UCHL1 antibody
Host
Chicken
Reactivity
Human, Mouse, Rat, Bovine, Porcine, Equine
Clonality
Polyclonal
Isotype
Chicken IgY
Form/Format
Liquid. Supplied as an aliquot of IgYpreparation plus 5mM NaN3
Applicable Applications for anti-PGP9.5/UCHL1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Recombinant full length human UCHL1 expressed in and purified from E coli.
Preparation and Storage
The antibody can be stored at 2 degree - 8 degree C for 12 month without detectable loss of activity. Avoidrepeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, AAA76716, dilution 1:2,000 in green, and mouse mAb to Actin in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties.)
WB (Western Blot)
(Western blot analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, AAA76716, dilution 1:2,000 in green, and mouse mAb to Actin in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties.)
IF (Immunofluorescence)
(Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to UCHL1, AAA76716, dilution 1:500 in red, and costained with mouse mAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells.)
IF (Immunofluorescence)
(Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to UCHL1, AAA76716, dilution 1:500 in red, and costained with mouse mAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells.)
Related Product Information for anti-PGP9.5/UCHL1 antibody
Ubiquitin C-terminal hydrolase 1 (UCHL1) is an extremely abundant protein of brain, where it is localized only in neurons. It was originally named PGP9.5 and discovered as a major protein spot on 2D gels of brain extracts which was absent on similar gels of other tissues. Later it was found that the PGP9.5 protein was an enzyme which could cleave ubiquitin monomers from ubiquitin conjugates and polyubiquitin chains, resulting in recycling of ubiquitin monomers and the renaming of PGP9.5 to UCHL1 to reflect this enzymatic activity. UCHL1 is an essential enzyme and defects in UCHL1 protein expression are involved in Parkinson's disease (PD) and other more serious disease states. In addition, UCHL1 may be released into cerebrospinal fluid (CSF) and blood following CNS damage and disease resulting in neuronal loss.
This antibody was made against full length recombinant human UCHL1 expressed in and purified from E coli and can be used to identify neurons and their processes in culture or in sections. The antibody works cleanly on appropriate lysates of cell and tissues. Considerable interest has been focused on the detection of UCHL1 in the blood and CSF of patients with traumatic injuries to the brain or spinal cord. This antibody has been widely used as both a capture and a detection reagent in ELISA type assays for measuring UCHL1 levels in blood and CSF samples.
This antibody was made against full length recombinant human UCHL1 expressed in and purified from E coli and can be used to identify neurons and their processes in culture or in sections. The antibody works cleanly on appropriate lysates of cell and tissues. Considerable interest has been focused on the detection of UCHL1 in the blood and CSF of patients with traumatic injuries to the brain or spinal cord. This antibody has been widely used as both a capture and a detection reagent in ELISA type assays for measuring UCHL1 levels in blood and CSF samples.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,824 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
UniProt Entry Name
UCHL1_HUMAN
Similar Products
Product Notes
The PGP9.5/UCHL1 uchl1 (Catalog #AAA76716) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The PGP9.5/UCHL1 reacts with Human, Mouse, Rat, Bovine, Porcine, Equine and may cross-react with other species as described in the data sheet. AAA Biotech's PGP9.5/UCHL1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the PGP9.5/UCHL1 uchl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGP9.5/UCHL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.