DB (Dot Blot)
(Dot blot analysis of CCNB1 Antibody (Phospho S35) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Rabbit anti-Human Phospho-CCNB1 (S35) Polyclonal Antibody | anti-CCNB1 antibody
Phospho-CCNB1 (S35) Antibody
Gene Names
CCNB1; CCNB
Reactivity
Human
Applications
Western Blot, ELISA, Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-CCNB1 (S35), Antibody; Phospho-CCNB1 (S35) Antibody; G2/mitotic-specific cyclin-B1; CCNB1; CCNB; anti-CCNB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.29 (varies by lot)
Sequence Length
396
Applicable Applications for anti-CCNB1 antibody
WB (Western Blot), ELISA, DB (Dot Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of CCNB1 Antibody (Phospho S35) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
DB (Dot Blot)
(Dot blot analysis of CCNB1 Antibody (Phospho S35) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
WB (Western Blot)
(Western blot analysis of lysate from Hela cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.AAA290418 was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of lysate from Hela cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.AAA290418 was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.)
Related Product Information for anti-CCNB1 antibody
The protein encoded by this gene is a regulatory protein
involved in mitosis. The gene product complexes with p34(cdc2) to
form the maturation-promoting factor (MPF). Two alternative
transcripts have been found, a constitutively expressed transcript
and a cell cycle-regulated transcript, that is expressed
predominantly during G2/M phase. The different transcripts result
from the use of alternate transcription initiation sites. [provided
by RefSeq].
involved in mitosis. The gene product complexes with p34(cdc2) to
form the maturation-promoting factor (MPF). Two alternative
transcripts have been found, a constitutively expressed transcript
and a cell cycle-regulated transcript, that is expressed
predominantly during G2/M phase. The different transcripts result
from the use of alternate transcription initiation sites. [provided
by RefSeq].
Product Categories/Family for anti-CCNB1 antibody
References
Kreis, N.N., et al. Oncogene 29(41):5591-5603(2010)
van Zon, W., et al. J. Cell Biol. 190(4):587-602(2010)
Harley, M.E., et al. EMBO J. 29(14):2407-2420(2010)
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Nantajit, D., et al. PLoS ONE 5 (8), E12341 (2010) :
van Zon, W., et al. J. Cell Biol. 190(4):587-602(2010)
Harley, M.E., et al. EMBO J. 29(14):2407-2420(2010)
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Nantajit, D., et al. PLoS ONE 5 (8), E12341 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48337
NCBI Official Full Name
G2/mitotic-specific cyclin-B1
NCBI Official Synonym Full Names
cyclin B1
NCBI Official Symbol
CCNB1
NCBI Official Synonym Symbols
CCNB
NCBI Protein Information
G2/mitotic-specific cyclin-B1
UniProt Protein Name
G2/mitotic-specific cyclin-B1
UniProt Gene Name
CCNB1
UniProt Synonym Gene Names
CCNB
UniProt Entry Name
CCNB1_HUMAN
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Product Notes
The CCNB1 ccnb1 (Catalog #AAA290418) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CCNB1 (S35) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-CCNB1 (S35) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, DB (Dot Blot). Researchers should empirically determine the suitability of the CCNB1 ccnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-CCNB1 (S35), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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