IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human Phospho-KDR/FLK1 (Y996) Polyclonal Antibody | anti-KDR antibody
Phospho-KDR/FLK1 (Y996) Antibody
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-KDR/FLK1 (Y996), Antibody; Phospho-KDR/FLK1 (Y996) Antibody; Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; KDR; Protein-tyrosine kinase receptor flk-1; CD309; FLK1; VEGFR2; anti-KDR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This KDR/FLK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y996 of human KDR/FLK1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.63 (varies by lot)
Sequence Length
1356
Applicable Applications for anti-KDR antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Anti-Phospho-KDR/FLK1-Y996 Pab is used in Western blot to detect Phospho-KDR/FLK1-Y996 in HMVEC cell line lysate.Endothelial cells were stimulated with 50ug/ml VEGF for 5min; 20ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with Phospho-KDR/FLK1-Y996 Antibody (AAA283770)diluted to 1:500 in 1% Milk/TBST.Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.)
WB (Western Blot)
(Anti-Phospho-KDR/FLK1-Y996 Pab is used in Western blot to detect Phospho-KDR/FLK1-Y996 in HMVEC cell line lysate.Endothelial cells were stimulated with 50ug/ml VEGF for 5min; 20ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with Phospho-KDR/FLK1-Y996 Antibody (AAA283770)diluted to 1:500 in 1% Milk/TBST.Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.)
Related Product Information for anti-KDR antibody
KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.
Product Categories/Family for anti-KDR antibody
References
Le Boeuf, F., et al., J. Biol. Chem. 279(37):39175-39185 (2004).
Lee, Y.K., et al., Blood 104(3):788-794 (2004).
Sulpice, E., et al., Eur. J. Biochem. 271(16):3310-3318 (2004).
Murdaca, J., et al., J. Biol. Chem. 279(25):26754-26761 (2004).
List, A.F., et al., Exp. Hematol. 32(6):526-535 (2004).
Lee, Y.K., et al., Blood 104(3):788-794 (2004).
Sulpice, E., et al., Eur. J. Biochem. 271(16):3310-3318 (2004).
Murdaca, J., et al., J. Biol. Chem. 279(25):26754-26761 (2004).
List, A.F., et al., Exp. Hematol. 32(6):526-535 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
151527
NCBI Official Full Name
vascular endothelial growth factor receptor 2
NCBI Official Synonym Full Names
kinase insert domain receptor
NCBI Official Symbol
KDR
NCBI Official Synonym Symbols
FLK1; CD309; VEGFR; VEGFR2
NCBI Protein Information
vascular endothelial growth factor receptor 2
UniProt Protein Name
Vascular endothelial growth factor receptor 2
UniProt Gene Name
KDR
UniProt Synonym Gene Names
FLK1; VEGFR2; VEGFR-2; FLK-1; KDR
UniProt Entry Name
VGFR2_HUMAN
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Product Notes
The KDR kdr (Catalog #AAA283770) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-KDR/FLK1 (Y996) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-KDR/FLK1 (Y996) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KDR kdr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-KDR/FLK1 (Y996), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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