DB (Dot Blot)
(Dot blot analysis of anti-phospho-OCT4-S236 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit Phospho-OCT4 (S236) Polyclonal Antibody | anti-POU5F1 antibody
Phospho-OCT4 (S236) Antibody
Gene Names
POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Pig)
Applications
ELISA, Dot Blot
Synonyms
Phospho-OCT4 (S236), Antibody; Phospho-OCT4 (S236) Antibody; POU domain; class 5; transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3; POU5F1; OCT3; OCT4; OTF3; anti-POU5F1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This OCT4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S236 of human OCT4.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
360
Applicable Applications for anti-POU5F1 antibody
ELISA, DB (Dot Blot)
Cellular Location
Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus. {ECO:0000250|UniProtKB:P20263, ECO:0000269|PubMed:18191611, ECO:0000269|PubMed:19274063, ECO:0000269|PubMed:23024368}
Tissue Location
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of anti-phospho-OCT4-S236 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-phospho-OCT4-S236 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Related Product Information for anti-POU5F1 antibody
OCT4 encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Product Categories/Family for anti-POU5F1 antibody
References
Narwani, K., et al. In Vitro Cell. Dev. Biol. Anim. 46 (3-4), 309-316 (2010)
Raya, A., et al. Nat Protoc 5(4):647-660(2010)
Firth, A.L., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 298 (4), L548-L557 (2010)
Raya, A., et al. Nat Protoc 5(4):647-660(2010)
Firth, A.L., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 298 (4), L548-L557 (2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
POU domain, class 5, transcription factor 1 isoform 1
NCBI Official Synonym Full Names
POU class 5 homeobox 1
NCBI Official Symbol
POU5F1
NCBI Official Synonym Symbols
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
NCBI Protein Information
POU domain, class 5, transcription factor 1
UniProt Protein Name
POU domain, class 5, transcription factor 1
UniProt Gene Name
POU5F1
UniProt Synonym Gene Names
OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
UniProt Entry Name
PO5F1_HUMAN
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Product Notes
The POU5F1 pou5f1 (Catalog #AAA285152) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-OCT4 (S236) Antibody reacts with Human (Predicted Reactivity: Bovine, Mouse, Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-OCT4 (S236) can be used in a range of immunoassay formats including, but not limited to, ELISA, DB (Dot Blot). Researchers should empirically determine the suitability of the POU5F1 pou5f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-OCT4 (S236), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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