Rabbit anti-Human Phospho-Raptor (S863) Polyclonal Antibody | anti-RPTOR antibody

Phospho-Raptor (S863) Antibody

Cat. Num. AAA289679

• Gene Names: RPTOR; KOG1; Mip1
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Phospho-Raptor (S863), Antibody; Phospho-Raptor (S863) Antibody; Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin (TOR)-scaffold protein; RPTOR; KIAA1303; RAPTOR; anti-RPTOR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Raptor Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S863 of human Raptor.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Applicable Applications for anti-RPTOR antibody: ELISA, DB (Dot Blot)
• Function: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates theprotein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.
• Cellular Location: Cytoplasm. Lysosome. Cytoplasmic granule. Note=Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.
• Tissue Location: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

DB (Dot Blot)

(Dot blot analysis of anti-raptor-pS863 Pab (RB13350) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-RPTOR antibody

Raptor participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). It plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. It has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, raptor serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions.

Product Categories/Family for anti-RPTOR antibody

Cell Biology; Signal Transduction; Phospho Antibodies

References

Wang,Y., Circ. Res. 101 (6), 560-569 (2007) Fuchs,B.C., Am. J. Physiol., Cell Physiol. 293 (1), C55-C63 (2007) Olsen,J.V., Cell 127 (3), 635-648 (2006)

NCBI and Uniprot Product Information

• NCBI GI #: 22094987
• NCBI GeneID: 57521
• NCBI Accession #: NP_065812.1
• NCBI GenBank Nucleotide #: NM_020761.2
• UniProt Accession #: Q8N122; Q8N4V9; Q8TB32; Q9P2P3; B2RN36; C6KEF2; F5H7J5
• Molecular Weight: 149038
• NCBI Official Full Name: regulatory-associated protein of mTOR isoform 1
• NCBI Official Synonym Full Names: regulatory associated protein of MTOR, complex 1
• NCBI Official Symbol: RPTOR
• NCBI Official Synonym Symbols: KOG1; Mip1
• NCBI Protein Information: regulatory-associated protein of mTOR
• UniProt Protein Name: Regulatory-associated protein of mTOR
• UniProt Gene Name: RPTOR
• UniProt Synonym Gene Names: KIAA1303; RAPTOR; Raptor
• UniProt Entry Name: RPTOR_HUMAN