Rabbit Phospho-Rb-like-1 (S975) Polyclonal Antibody | anti-RBL1 antibody

Phospho-Rb-like-1 (S975) Antibody

Cat. Num. AAA290145

• Gene Names: RBL1; PRB1; p107; CP107
• Reactivity: Reactivity: Human; Predicted Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Phospho-Rb-like-1 (S975), Antibody; Phospho-Rb-like-1 (S975) Antibody; Retinoblastoma-like protein 1; 107 kDa retinoblastoma-associated protein; p107; pRb1; RBL1; anti-RBL1 antibody

• Host: Rabbit
• Reactivity: Reactivity: Human; Predicted Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Rb-like-1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S975 of human Rb-like-1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 1,014

• Applicable Applications for anti-RBL1 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Function: Key regulator of entry into cell division (PubMed: ). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation (By similarity). Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression (By similarity). Controls histone H4 'Lys-20' trimethylation (By similarity). Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters (By similarity). Potent inhibitor of E2F-mediated trans-activation (PubMed: ). May act as a tumor suppressor (PubMed: ).
• Cellular Location: Nucleus.
• Preparation and Storage: Maintain refrigerated at 2°C to 8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(The anti-Phospho-Rb-like-1-S975 Pab AAA290145 is used in Western blot to detect Phospho-Rb-like-1-S975 in A2058 tissue lysate)

WB (Western Blot)

(All lanes : Anti-Phospho-Rb-like-1(S975) Antibody (upper) at 1:250 dilution + Hela whole cell lysates, Beta-actin (lower)Lane 1: G0-phase?serum-deprived 24h?Lane 2: S-phase?hydroxycarbamide?5mM, 24hLane 3: G2/M-phase?etoposide?5mM, 24hLane 4: M-phase, nocodazole, 150ng/ml 20hLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 121 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RBL1 antibody

RB-like 1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and RB-like 1 can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that RB-like 1 may also be a tumor suppressor.

Product Categories/Family for anti-RBL1 antibody

Cancer; Cell Biology; Signal Transduction; Phospho Antibodies

References

Rodier, G., et al., J. Cell Biol. 168(1):55-66 (2005).
Barbie, T.U., et al., Proc. Natl. Acad. Sci. U.S.A. 100(26):15601-15606 (2003).
Joaquin, M., et al., J. Biol. Chem. 278(45):44255-44264 (2003).
Cicchillitti, L., et al., J. Biol. Chem. 278(21):19509-19517 (2003).
Leng, X., et al., Mol. Cell. Biol. 22(7):2242-2254 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 34577079
• NCBI GeneID: 5933
• NCBI Accession #: NP_002886.2; NP_899662.1
• NCBI GenBank Nucleotide #: NM_002895.3
• UniProt Accession #: P28749; Q4VXA0; Q8N5K6; Q9H1L5; Q9H1M1; A8K2W5
• NCBI Official Full Name: retinoblastoma-like protein 1 isoform a
• NCBI Official Synonym Full Names: retinoblastoma-like 1
• NCBI Official Symbol: RBL1
• NCBI Official Synonym Symbols: PRB1; p107; CP107
• NCBI Protein Information: retinoblastoma-like protein 1
• UniProt Protein Name: Retinoblastoma-like protein 1
• UniProt Gene Name: RBL1
• UniProt Synonym Gene Names: p107
• UniProt Entry Name: RBL1_HUMAN