DB (Dot Blot)
(Dot blot analysis of ULK1 Antibody (Phospho S556) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Rabbit anti-Human Phospho-ULK1 (S556) Polyclonal Antibody | anti-ULK1 antibody
Phospho-ULK1 (S556) Antibody
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Reactivity
Human
Applications
ELISA, Dot Blot, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-ULK1 (S556), Antibody; Phospho-ULK1 (S556) Antibody; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; ATG1; hATG1; Unc-51-like kinase 1; ULK1; KIAA0722; anti-ULK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ULK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S556 of human ULK1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
1,050
Applicable Applications for anti-ULK1 antibody
ELISA, DB (Dot Blot), IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of ULK1 Antibody (Phospho S556) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
DB (Dot Blot)
(Dot blot analysis of ULK1 Antibody (Phospho S556) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with ULK1 (phospho S556) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA287037 ULK1 (phospho S556) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ULK1 (phospho S556) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with ULK1 (phospho S556) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA287037 ULK1 (phospho S556) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ULK1 (phospho S556) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Related Product Information for anti-ULK1 antibody
Involved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an essential role in neurite extension of cerebellar granule cells (By similarity).
Product Categories/Family for anti-ULK1 antibody
References
References for protein:
1.Mercer, C.A., et al. Autophagy 5(5):649-662(2009)
2.Ganley, I.G., et al. J. Biol. Chem. 284(18):12297-12305(2009)
3.Jung, C.H., et al. Mol. Biol. Cell 20(7):1992-2003(2009)
4.Hosokawa, N., et al. Mol. Biol. Cell 20(7):1981-1991(2009)
5.Chan, E.Y. Sci Signal 2 (84), PE51 (2009)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].
1.Mercer, C.A., et al. Autophagy 5(5):649-662(2009)
2.Ganley, I.G., et al. J. Biol. Chem. 284(18):12297-12305(2009)
3.Jung, C.H., et al. Mol. Biol. Cell 20(7):1992-2003(2009)
4.Hosokawa, N., et al. Mol. Biol. Cell 20(7):1981-1991(2009)
5.Chan, E.Y. Sci Signal 2 (84), PE51 (2009)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112631
NCBI Official Full Name
serine/threonine-protein kinase ULK1
NCBI Official Synonym Full Names
unc-51 like autophagy activating kinase 1
NCBI Official Symbol
ULK1
NCBI Official Synonym Symbols
ATG1; ATG1A; UNC51; hATG1; Unc51.1
NCBI Protein Information
serine/threonine-protein kinase ULK1
UniProt Protein Name
Serine/threonine-protein kinase ULK1
UniProt Gene Name
ULK1
UniProt Synonym Gene Names
KIAA0722; ATG1; hATG1
UniProt Entry Name
ULK1_HUMAN
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Product Notes
The ULK1 ulk1 (Catalog #AAA287037) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ULK1 (S556) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-ULK1 (S556) can be used in a range of immunoassay formats including, but not limited to, ELISA, DB (Dot Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the ULK1 ulk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-ULK1 (S556), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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