IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with hPI3KC3 (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit PI3KC3 Polyclonal Antibody | anti-PIK3C3 antibody
PI3KC3 Antibody (C-term E785)
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PI3KC3, Antibody; PI3KC3 Antibody (C-term E785); Phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34; PIK3C3; VPS34; anti-PIK3C3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Clonality
Polyclonal
Specificity
This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-801 amino acids from the C-terminal region of human PI3KC3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
770-801
Sequence Length
887
Applicable Applications for anti-PIK3C3 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Function
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol3-phosphate; different complex forms arebelieved to play a role in multiple membranetrafficking pathways: PI3KC3-C1 is involved ininitiation of autophagosomes and PI3KC3-C2in maturation of autophagosomes andendocytosis. Involved in regulation ofdegradative endocytic trafficking andrequired for the abcission step in cytokinesis,probably in the context of PI3KC3-C2.
Cellular location
Midbody. Late endosome. Cytoplasmicvesicle, autophagosome. Note=As componentof the PI3K complex I localized topre-autophagosome structures. Ascomponent of the PI3K complex II localizedpredominantly to endosomes (Probable).Localizes also to discrete punctae along theciliary axoneme and to the base of the ciliaryaxoneme .
Tissue location
Ubiquitously expressed, with a highestexpression in skeletal muscle.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with hPI3KC3 (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with hPI3KC3 (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysate from U-251 MG cell line, using hPI3KC3 (C-term). AAA288963 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from U-251 MG cell line, using hPI3KC3 (C-term). AAA288963 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Related Product Information for anti-PIK3C3 antibody
PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.
Product Categories/Family for anti-PIK3C3 antibody
References
Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003).
Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).
Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
phosphatidylinositol 3-kinase catalytic subunit type 3 isoform 1
NCBI Official Synonym Full Names
phosphatidylinositol 3-kinase, catalytic subunit type 3
NCBI Official Symbol
PIK3C3
NCBI Official Synonym Symbols
VPS34; Vps34; hVps34
NCBI Protein Information
phosphatidylinositol 3-kinase catalytic subunit type 3
UniProt Protein Name
Phosphatidylinositol 3-kinase catalytic subunit type 3
UniProt Gene Name
PIK3C3
UniProt Synonym Gene Names
VPS34; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3
UniProt Entry Name
PK3C3_HUMAN
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Product Notes
The PIK3C3 pik3c3 (Catalog #AAA288963) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 770-801. The PI3KC3 Antibody (C-term E785) reacts with Human (Predicted Reactivity: Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PI3KC3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PIK3C3 pik3c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3KC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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