FCM/FACS (Flow Cytometry)
(PIK3R5 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human PIK3R5 Polyclonal Antibody | anti-PIK3R5 antibody
PIK3R5 Antibody (C-term)
Gene Names
PIK3R5; p101; FOAP-2; P101-PI3K; F730038I15Rik
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PIK3R5, Antibody; PIK3R5 Antibody (C-term); Phosphoinositide 3-kinase regulatory subunit 5; PI3-kinase regulatory subunit 5; PI3-kinase p101 subunit; Phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit; PtdIns-3-kinase regulatory subunit; Protein FOAP-2; PtdIns-3-kinase p101; p101-PI3K; PIK3R5; anti-PIK3R5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PIK3R5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 763-792 amino acids from the C-terminal region of human PIK3R5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.46 (varies by lot)
Sequence Positions
763-792
Sequence Length
880
Applicable Applications for anti-PIK3R5 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma membrane via interaction with beta-gamma G protein dimers. Required for G protein-mediated activation of PIK3CG (By similarity).
Cellular Location
Nucleus {ECO:0000250|UniProtKB:O02696}. Cytoplasm {ECO:0000250|UniProtKB:O02696}. Cell membrane {ECO:0000250|UniProtKB:O02696}; Peripheral membrane protein {ECO:0000250|UniProtKB:O02696}. Note=Predominantly localized in the nucleus in absence of PIK3CG/p120. Colocalizes with PIK3CG/p120 in the cytoplasm. Translocated to the plasma membrane in a beta-gamma G protein-dependent manner. {ECO:0000250|UniProtKB:O02696}
Tissue Location
Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli
Preparation and Storage
Maintain refrigerated at 2-8°C up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PIK3R5 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PIK3R5 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(PIK3R5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIK3R5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PIK3R5 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIK3R5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PIK3R5 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the PIK3R5 antibody detected the PIK3R5 protein (arrow).)
WB (Western Blot)
(PIK3R5 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the PIK3R5 antibody detected the PIK3R5 protein (arrow).)
Related Product Information for anti-PIK3R5 antibody
Receptor-regulated class I phosphoinositide 3-kinases
(PI3Ks) phosphorylate the membrane lipid phosphatidylinositol
4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turn
recruits and activates cytosolic effectors involved in
proliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatory
subunit (Brock et al., 2003 [PubMed 12507995]).
(PI3Ks) phosphorylate the membrane lipid phosphatidylinositol
4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turn
recruits and activates cytosolic effectors involved in
proliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatory
subunit (Brock et al., 2003 [PubMed 12507995]).
Product Categories/Family for anti-PIK3R5 antibody
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Segat, L., et al. Vaccine 28(10):2201-2206(2010)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Johnson, C., et al. Oncogene 26(49):7049-7057(2007)
Suire, S., et al. Curr. Biol. 15(6):566-570(2005)
Segat, L., et al. Vaccine 28(10):2201-2206(2010)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Johnson, C., et al. Oncogene 26(49):7049-7057(2007)
Suire, S., et al. Curr. Biol. 15(6):566-570(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97348
NCBI Official Full Name
phosphoinositide 3-kinase regulatory subunit 5 isoform 1
NCBI Official Synonym Full Names
phosphoinositide-3-kinase, regulatory subunit 5
NCBI Official Symbol
PIK3R5
NCBI Official Synonym Symbols
p101; FOAP-2; P101-PI3K; F730038I15Rik
NCBI Protein Information
phosphoinositide 3-kinase regulatory subunit 5
UniProt Protein Name
Phosphoinositide 3-kinase regulatory subunit 5
UniProt Gene Name
PIK3R5
UniProt Synonym Gene Names
PI3-kinase regulatory subunit 5; PtdIns-3-kinase regulatory subunit
UniProt Entry Name
PI3R5_HUMAN
Similar Products
Product Notes
The PIK3R5 pik3r5 (Catalog #AAA285763) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 763-792. The PIK3R5 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIK3R5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PIK3R5 pik3r5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3R5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.