Rabbit anti-Human, Mouse PKCdelta Polyclonal Antibody | anti-PRKCD antibody

PRKCD Polyclonal Antibody

Cat. Num. AAA36743

• Gene Names: PRKCD; MAY1; PKCD; nPKC-delta
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Affinity purification
• Synonyms: PKCdelta, Antibody; PRKCD Polyclonal Antibody; PRKCD; PKCd ; MAY1; MGC49908; PKCD; nPKC-delta; Protein kinase C delta type ; PKCdelta;; anti-PRKCD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
• Sequence Length: 676

• Applicable Applications for anti-PRKCD antibody: FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic -peptide of human PRKCD
• Preparation and Storage: Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded human esophagus using PRKCD antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human liver cancer using PRKCD antibody at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using PRKCD antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Related Product Information for anti-PRKCD antibody

Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calciumdependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calciumin-dependent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation at Thr500 in the activation loop, the autophosphorylation site at Thr641, and at carboxyterminal hydrophobic site Ser660 occurs in vivo (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. Either the enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCu (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKCrelated kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7).

References

1. Nishizuka, Y. (1984) Nature 308, 693-698. 2. Keranen, L.M. et al. (1995) Curr. Biol. 5, 1394-1403. 3. Mellor, H. and Parker, P.J. (1998) Biochem J. 332 (Pt 2), 281-292. 4. Ron, D. and Kazanietz, M.G. (1999) FASEB J. 13, 1658-1676. 5. Moscat, J. and DiazMeco, M.T. (2000) EMBO Rep. 1, 399-403. 6. Baron, C.L. and Malhotra, V. (2002) Science 295, 325-328. 7. Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-11070.

NCBI and Uniprot Product Information

• NCBI GI #: 31377782
• NCBI GeneID: 5580
• NCBI Accession #: NP_006245.2
• NCBI GenBank Nucleotide #: NM_006254.3
• UniProt Accession #: Q05655; Q15144; Q86XJ6; B0KZ81; B2R834
• Molecular Weight: 78kDa
• NCBI Official Full Name: protein kinase C delta type
• NCBI Official Synonym Full Names: protein kinase C, delta
• NCBI Official Symbol: PRKCD
• NCBI Official Synonym Symbols: MAY1; PKCD; nPKC-delta
• NCBI Protein Information: protein kinase C delta type; protein kinase C delta VIII; tyrosine-protein kinase PRKCD
• UniProt Protein Name: Protein kinase C delta type
• UniProt Gene Name: PRKCD
• UniProt Synonym Gene Names: SDK1
• UniProt Entry Name: KPCD_HUMAN