IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human PKLR Polyclonal Antibody | anti-PKLR antibody
PKLR Antibody (N-term)
Gene Names
PKLR; PK1; PKL; PKR; RPK; PKRL
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PKLR, Antibody; PKLR Antibody (N-term); Pyruvate kinase PKLR; Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase; PKLR; PK1; PKL; anti-PKLR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PKLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PKLR.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1-30
Sequence Length
543
Applicable Applications for anti-PKLR antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(PKLR Antibody (S8) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PKLR antibody detected the PKLR protein (arrow).)
WB (Western Blot)
(PKLR Antibody (S8) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PKLR antibody detected the PKLR protein (arrow).)
WB (Western Blot)
(The anti-PKLR Pab is used in Western blot to detect PKLR in NIH/3T3 cell lysate.)
WB (Western Blot)
(The anti-PKLR Pab is used in Western blot to detect PKLR in NIH/3T3 cell lysate.)
Related Product Information for anti-PKLR antibody
PKLR is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).
Product Categories/Family for anti-PKLR antibody
References
Wang, H., et al., Diabetes 51(9):2861-2865 (2002). Valentini, G., et al., J. Biol. Chem. 277(26):23807-23814 (2002). Mateu, E., et al., Hum. Genet. 110(6):532-544 (2002). Indo, Y., et al., Hum. Mutat. 18(4):308-318 (2001). Acan, N.L., et al., J. Enzym. Inhib. 16(5):457-464 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61830
NCBI Official Full Name
pyruvate kinase PKLR isoform 1
NCBI Official Synonym Full Names
pyruvate kinase, liver and RBC
NCBI Official Symbol
PKLR
NCBI Official Synonym Symbols
PK1; PKL; PKR; RPK; PKRL
NCBI Protein Information
pyruvate kinase PKLR
UniProt Protein Name
Pyruvate kinase PKLR
UniProt Gene Name
PKLR
UniProt Synonym Gene Names
PK1; PKL
UniProt Entry Name
KPYR_HUMAN
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Product Notes
The PKLR pklr (Catalog #AAA288937) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The PKLR Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PKLR can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PKLR pklr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKLR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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