WB (Western Blot)
(Detection of human PLA2G4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLA2G4A antibody AAA213148 (lot AAA213148-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human PLA2G4A/cPLA2 Polyclonal Antibody | anti-PLA2G4A/cPLA2 antibody
Rabbit anti-PLA2G4A/cPLA2 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PLA2G4A/cPLA2, Antibody; Rabbit anti-PLA2G4A/cPLA2 Antibody, Affinity Purified; cPLA2-alpha; PLA2G4; phospholipase A2, group IVA (cytosolic, calcium-dependent); Phospholipase A2 group IVA; Phospholipase A2; phosphatidylcholine 2-acylhydrolase; cytosolic phospholipase A2; cPLA2; calcium-dependent phospholipid-binding protein; lysophospholipase; Cytosolic phospholipase A2; phospholipase A2 group IVA; anti-PLA2G4A/cPLA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PLA2G4A/cPLA2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 699 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PLA2G4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLA2G4A antibody AAA213148 (lot AAA213148-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human PLA2G4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLA2G4A antibody AAA213148 (lot AAA213148-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PLA2G4A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLA2G4A antibody AAA213148 (lot AAA213148-1) used for IP at 6 ug per reaction. PLA2G4A was also immunoprecipitated by rabbit anti-PLA2G4A antibody For blotting immunoprecipitated PLA2G4A, AAA213148 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PLA2G4A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLA2G4A antibody AAA213148 (lot AAA213148-1) used for IP at 6 ug per reaction. PLA2G4A was also immunoprecipitated by rabbit anti-PLA2G4A antibody For blotting immunoprecipitated PLA2G4A, AAA213148 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-PLA2G4A/cPLA2 antibody
The gene PLA2G4A encodes a member of the cytosolic phospholipase A2 group IV family, cytosolic phospholipase A2. This enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate inflammatory responses. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor [taken from NCBI Entrez Gene (Gene ID: 5321)].
Product Categories/Family for anti-PLA2G4A/cPLA2 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PLA2G4A/cPLA2 pla2g4a (Catalog #AAA213148) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PLA2G4A/cPLA2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G4A/cPLA2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PLA2G4A/cPLA2 pla2g4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLA2G4A/cPLA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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