PLK1 Polyclonal Antibody | anti-PLK1 antibody

Anti-PLK1 Antibody

Cat. Num. AAA46506

• Gene Names: PLK1; PLK; STPK13
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: PLK1, Antibody; Anti-PLK1 Antibody; Serine/threonine-protein kinase PLK1; Cell cycle regulated protein kinase; PLK 1; PLK; PLK-1; plk1; PLK1_HUMAN; Polo like kinase 1; Polo-like kinase 1; Serine/threonine protein kinase 13; Serine/threonine protein kinase PLK1; Serine/threonine-protein kinase 13; STPK 13; STPK13; polo-like kinase 1; anti-PLK1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 603

• Applicable Applications for anti-PLK1 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived human PLK1 recombinant protein (Position: K86-N430). Human PLK1 shares 95.4% and 96.2% amino acid (aa) sequence identity with mouse and rat PLK1, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

IHC (Immunohistochemistry)

(Anti- PLK1 Picoband antibody, AAA46506,IHC(P)IHC(P): Human Lung Cancer Tissue)

IHC (Immunohistochemisry)

(Anti- PLK1 Picoband antibody, AAA46506,IHC(P)IHC(P): Rat Lymphaden Tissue)

IHC (Immunohiostchemistry)

(Anti- PLK1 Picoband antibody, AAA46506,IHC(P)IHC(P): Mouse Lymphaden Tissue)

WB (Western Blot)

(Anti- PLK1 Picoband antibody, AAA46506, Western blottingAll lanes: Anti PLK1 (AAA46506) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: PANC Whole Cell Lysate at 40ugPredicted bind size: 68KDObserved bind size: 68KD)

Related Product Information for anti-PLK1 antibody

Description: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase PLK1(PLK1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13(STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth. Based on yeast and murine studies of meiosis, human PLK1 may also have a regulatory function in meiosis.

References

1. Holtrich U, Wolf G, Bräuninger A, Karn T, Böhme B, Rübsamen-Waigmann H et al. (March 1994). "Induction and down-regulation of PLK, a human serine/threonine kinase expressed in proliferating cells and tumors". Proc. Natl. Acad. Sci. U.S.A. 91(5): 1736-40. 2. Soung NK, Park JE, Yu LR, Lee KH, Lee JM, Bang JK et al. (April 2009). "Plk1-dependent and -independent roles of an ODF2 splice variant, hCenexin1, at the centrosome of somatic cells". Dev. Cell 16(4): 539-50.

NCBI and Uniprot Product Information

• NCBI GI #: 21359873
• NCBI GeneID: 5347
• NCBI Accession #: NP_005021.2
• NCBI GenBank Nucleotide #: NM_005030.5
• UniProt Accession #: P53350; Q15153; Q99746
• Molecular Weight: 68,255 Da
• NCBI Official Full Name: serine/threonine-protein kinase PLK1
• NCBI Official Synonym Full Names: polo like kinase 1
• NCBI Official Symbol: PLK1
• NCBI Official Synonym Symbols: PLK; STPK13
• NCBI Protein Information: serine/threonine-protein kinase PLK1
• UniProt Protein Name: Serine/threonine-protein kinase PLK1
• UniProt Gene Name: PLK1
• UniProt Synonym Gene Names: PLK; PLK-1; STPK13
• UniProt Entry Name: PLK1_HUMAN