WB (Western Blot)
(Detection of human Pol Iota by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pol Iota antibody AAA211408 (lot AAA211408-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
Rabbit anti-Human Pol Iota Polyclonal Antibody | anti-PolIota antibody
Rabbit anti-Pol Iota Antibody, Affinity Purified
Gene Names
POLI; RAD30B; RAD3OB
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Pol Iota, Antibody; Rabbit anti-Pol Iota Antibody, Affinity Purified; polymerase (DNA) iota; RAD3OB; RAD30B; polymerase (DNA-directed), iota; polymerase (DNA directed) iota; eta2; DNA polymerase iota; RAD30 homolog B; anti-PolIota antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PolIota antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Monkey, Gorilla, Chimpanzee
Immunogen
Between 690 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Pol Iota by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pol Iota antibody AAA211408 (lot AAA211408-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Pol Iota by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pol Iota antibody AAA211408 (lot AAA211408-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Pol Iota by western blot of immunoprecipitaes. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pol Iota antibody AAA211408 (lot AAA211408-1) used for IP at 3 ug/mg lysate. Pol Iota was also immunoprecipitated by rabbit anti-Pol Iota antibody which recognizes a downstream epitope. For blotting immunoprecipitated Pol Iota, AAA211408 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Pol Iota by western blot of immunoprecipitaes. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pol Iota antibody AAA211408 (lot AAA211408-1) used for IP at 3 ug/mg lysate. Pol Iota was also immunoprecipitated by rabbit anti-Pol Iota antibody which recognizes a downstream epitope. For blotting immunoprecipitated Pol Iota, AAA211408 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
Related Product Information for anti-PolIota antibody
Pol Iota is a DNA polymerase involved in DNA repair and translesion DNA synthesis. Pol Iota is a member of the Rad30 family of DNA polymerases which exhibit low fidelity when replicating undamaged DNA, and in this context may play a role in the hypermutation of immunoglobulin genes. Pol Iota is also known as RAD30B and is a paralog of RAD30A, also known as Pol eta.
Product Categories/Family for anti-PolIota antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,006 Da
NCBI Official Full Name
DNA polymerase iota
NCBI Official Synonym Full Names
polymerase (DNA directed) iota
NCBI Official Symbol
POLI
NCBI Official Synonym Symbols
RAD30B; RAD3OB
NCBI Protein Information
DNA polymerase iota; eta2; RAD30 homolog B; polymerase (DNA-directed), iota
UniProt Protein Name
DNA polymerase iota
UniProt Gene Name
POLI
UniProt Synonym Gene Names
RAD30B
UniProt Entry Name
POLI_HUMAN
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Product Notes
The PolIota poli (Catalog #AAA211408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Pol Iota Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pol Iota can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PolIota poli for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pol Iota, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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