WB (Western Blot)
(RNA pol II antibody tested by Western blot. RNA pol II detection by Western blot analysis. RNA pol II is detected in HeLa nuclear extracts using RNA pol II mouse monoclonal antibody at a 1:2,000 dilution.)
Mouse RNA pol II Monoclonal Antibody | anti-RNApolII antibody
RNA pol II antibody (mAb)(Clone 4H8)
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Budding Yeast, C. elegans, Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
RNA pol II, Antibody; RNA pol II antibody (mAb)(Clone 4H8); RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220; anti-RNApolII antibody
Host
Mouse
Reactivity
Budding Yeast, C. elegans, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4H8
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.2% gelatin, 0.01% thimerosal and 0.1% sodium azide. Thimerosal and sodium azide are highly toxic.
Concentration
0.2ug/ul (varies by lot)
Applicable Applications for anti-RNApolII antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This RNA pol II antibody was raised against a synthetic peptide YSPTSpPS corresponding to human RNA pol II. It recognizes both the phosphorylated and unphosphorylated forms of the largest subunit of RNA pol II.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(RNA pol II antibody tested by Western blot. RNA pol II detection by Western blot analysis. RNA pol II is detected in HeLa nuclear extracts using RNA pol II mouse monoclonal antibody at a 1:2,000 dilution.)
WB (Western Blot)
(RNA pol II antibody tested by Western blot. RNA pol II detection by Western blot analysis. RNA pol II is detected in HeLa nuclear extracts using RNA pol II mouse monoclonal antibody at a 1:2,000 dilution.)
ChIP (Chromatin Immunoprecipitation)
(RNA pol II antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 2.3 million HL-60 cells and 20 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify Pol II binding. ChIP-Seq data from three specific genes is shown as an example. Pol II binding is detected at the 5´ end of the genes, throughout the gene bodies and at the 3´ end of the genes.)
ChIP (Chromatin Immunoprecipitation)
(RNA pol II antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 2.3 million HL-60 cells and 20 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify Pol II binding. ChIP-Seq data from three specific genes is shown as an example. Pol II binding is detected at the 5´ end of the genes, throughout the gene bodies and at the 3´ end of the genes.)
Related Product Information for anti-RNApolII antibody
Short Description: Our RNA pol II antibody (mAb)(Clone 4H8) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Budding Yeast, C. elegans, Human, Mouse and Rat samples.
Background: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Background: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Product Categories/Family for anti-RNApolII antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,641 Da
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB1
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
NCBI Official Symbol
POLR2A
NCBI Official Synonym Symbols
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA polymerase II subunit B1; RNA-dir
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB1
UniProt Gene Name
POLR2A
UniProt Synonym Gene Names
POLR2; RNA polymerase II subunit B1
UniProt Entry Name
RPB1_HUMAN
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Product Notes
The RNApolII polr2a (Catalog #AAA59824) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RNA pol II antibody (mAb)(Clone 4H8) reacts with Budding Yeast, C. elegans, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RNA pol II can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the RNApolII polr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA pol II, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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