WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: HCT116) using a 5% SDS PAGE gel and POLR3A antibody at a dilution of 1:500)
Rabbit POLR3A Polyclonal Antibody | anti-POLR3A antibody
POLR3A Antibody
Gene Names
POLR3A; ADDH; HLD7; RPC1; WDRTS; RPC155; hRPC155
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Antigen-Affinity Chromatography
Synonyms
POLR3A, Antibody; POLR3A Antibody; Affinity Purified Rabbit Polyclonal POLR3A Antibody; Polyclonal POLR3A antibody; Anti-POLR3A antibody; hRPC155 antibody; RNA polymerase III subunit RPC155-D antibody; POLRA-3; POLRA 3; POLR3A; RNA polymerase III subunit C160 antibody; POLRA 3 antibody; RPC155 antibody; DNA-directed RNA polymerase III subunit RPC1 antibody; POLR3A antibody; RPC1 antibody; POLRA-3 antibody; DNA-directed RNA polymerase III subunit A antibody; anti-POLR3A antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.89 mg/ml (varies by lot)
Sequence Length
162
Applicable Applications for anti-POLR3A antibody
WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
POLR3A antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 182 and 482 of POLR3A
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: HCT116) using a 5% SDS PAGE gel and POLR3A antibody at a dilution of 1:500)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: HCT116) using a 5% SDS PAGE gel and POLR3A antibody at a dilution of 1:500)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using POLR3A antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
IF (Immunofluorescence)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using POLR3A antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Related Product Information for anti-POLR3A antibody
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
Product Categories/Family for anti-POLR3A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
156 kDa
NCBI Official Full Name
POLR3A protein
NCBI Official Synonym Full Names
RNA polymerase III subunit A
NCBI Official Symbol
POLR3A
NCBI Official Synonym Symbols
ADDH; HLD7; RPC1; WDRTS; RPC155; hRPC155
NCBI Protein Information
DNA-directed RNA polymerase III subunit RPC1
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Product Notes
The POLR3A (Catalog #AAA225306) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's POLR3A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the POLR3A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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