WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysateAll lanes: POU4F1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43, 34 kDaObserved band size: 43 kDa)
Rabbit anti-Human POU4F1 Polyclonal Antibody | anti-POU4F1 antibody
POU4F1 Antibody
Gene Names
POU4F1; BRN3A; RDC-1; Oct-T1; brn-3A
Reactivity
Human
Applications
Immunofluorescence, Western Blot, ELISA
Purity
>95%, Protein G Purified
Synonyms
POU4F1, Antibody; POU4F1 Antibody; POU domain; class 4; Transcription factor 1 (Brain-specific homeobox/POU domain protein 3A) (Brain-3A) (Brn-3A) (Homeobox/POU domain protein RDC-1) (Oct-T1); POU4F1; BRN3A RDC1; anti-POU4F1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G Purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Applicable Applications for anti-POU4F1 antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen
Recombinant Human POU domain, class 4, transcription factor 1 protein (258-419AA)
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysateAll lanes: POU4F1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43, 34 kDaObserved band size: 43 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysateAll lanes: POU4F1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43, 34 kDaObserved band size: 43 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells with AAA243432 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells with AAA243432 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Product Categories/Family for anti-POU4F1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42 KD
NCBI Official Full Name
POU domain, class 4, transcription factor 1
NCBI Official Synonym Full Names
POU class 4 homeobox 1
NCBI Official Symbol
POU4F1
NCBI Official Synonym Symbols
BRN3A; RDC-1; Oct-T1; brn-3A
NCBI Protein Information
POU domain, class 4, transcription factor 1; brain-3A; homeobox/POU domain protein RDC-1; brain-specific homeobox/POU domain protein 3A
UniProt Protein Name
POU domain, class 4, transcription factor 1
UniProt Gene Name
POU4F1
UniProt Synonym Gene Names
BRN3A; RDC1; Brain-3A; Brn-3A
UniProt Entry Name
PO4F1_HUMAN
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Product Notes
The POU4F1 pou4f1 (Catalog #AAA243432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POU4F1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POU4F1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the POU4F1 pou4f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU4F1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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