WB (Western Blot)
(Western blot analysis of lysate from SW480 cell line,using POU4F1 Antibody(AAA287061). AAA287061 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)
Rabbit POU4F1 Polyclonal Antibody | anti-POU4F1 antibody
POU4F1 Antibody
Gene Names
POU4F1; BRN3A; RDC-1; Oct-T1; brn-3A
Reactivity
Human, mouse, rat
Applications
Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
POU4F1, Antibody; POU4F1 Antibody; POU domain; class 4; transcription factor 1; Brain-specific homeobox/POU domain protein 3A; Brain-3A; Brn-3A; Homeobox/POU domain protein RDC-1; Oct-T1; POU4F1; BRN3A; RDC1; anti-POU4F1 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol.
Sequence Positions
321-349
Sequence Length
419
Applicable Applications for anti-POU4F1 antibody
WB (Western Blot)
Antigen Type
Synthetic Peptide
Function
Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'- ATGCAAAT-3'in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site.
Cellular Location
Nucleus. Cytoplasm {ECO:0000250|UniProtKB:P17208} Tissue
Location
Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.
Preparation and Storage
Store at -20°C.
WB (Western Blot)
(Western blot analysis of lysate from SW480 cell line,using POU4F1 Antibody(AAA287061). AAA287061 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from SW480 cell line,using POU4F1 Antibody(AAA287061). AAA287061 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)
Related Product Information for anti-POU4F1 antibody
Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Product Categories/Family for anti-POU4F1 antibody
References
Bhargava A.K.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:10260-10264(1993).
Xiang M.,et al.J. Neurosci. 15:4762-4785(1995).
Dunham A.,et al.Nature 428:522-528(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Collum R.G.,et al.Nucleic Acids Res. 20:4919-4925(1992).
Xiang M.,et al.J. Neurosci. 15:4762-4785(1995).
Dunham A.,et al.Nature 428:522-528(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Collum R.G.,et al.Nucleic Acids Res. 20:4919-4925(1992).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43 kDa
NCBI Official Full Name
POU domain, class 4, transcription factor 1
NCBI Official Synonym Full Names
POU class 4 homeobox 1
NCBI Official Symbol
POU4F1
NCBI Official Synonym Symbols
BRN3A; RDC-1; Oct-T1; brn-3A
NCBI Protein Information
POU domain, class 4, transcription factor 1
UniProt Protein Name
POU domain, class 4, transcription factor 1
UniProt Gene Name
POU4F1
UniProt Synonym Gene Names
BRN3A; RDC1; Brain-3A; Brn-3A
UniProt Entry Name
PO4F1_HUMAN
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Product Notes
The POU4F1 pou4f1 (Catalog #AAA287061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 321-349. The POU4F1 Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's POU4F1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the POU4F1 pou4f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU4F1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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